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园艺学报 ›› 2014, Vol. 41 ›› Issue (6): 1125-1132.

• 蔬菜 • 上一篇    下一篇

葡聚六糖对菜薹立枯病和相关蛋白、内源激素的影响

康云艳,刘 兵,杨 暹*,柴喜荣   

  1. (华南农业大学园艺学院,广州 510642)
  • 收稿日期:2013-12-24 出版日期:2014-06-25 发布日期:2014-06-25

Effects of Glucohexaose on Sheath Blight,Pathogenesis-related Proteins and Endogenous Hormones in Flowering Chinese Cabbage

KANG Yun-yan,LIU Bing,YANG Xian*,and CHAI Xi-rong   

  1. (College of Horticulture,South China Agricultural University,Guangzhou 510642,China)
  • Received:2013-12-24 Online:2014-06-25 Published:2014-06-25

摘要: 通过不同浓度葡聚六糖和接种立枯病菌处理,研究了葡聚六糖对菜薹接种立枯病菌后的发病率、病情相关蛋白(PRs)和内源激素含量的影响。结果表明:葡聚六糖处理可提高菜薹对立枯病菌的抗性,降低发病率。葡聚六糖处理对菜薹产量没有明显的影响,而接种立枯病菌后,产量明显降低,但葡聚六糖处理可以抑制立枯病菌对菜薹生长的影响,减轻产量的损失。葡聚六糖和立枯病菌处理都能明显诱导提高菜薹植株体内病情相关蛋白几丁质酶、β–1,3–葡聚糖酶和内源激素乙烯、吲哚乙酸(IAA)、脱落酸(ABA)的水平,但葡聚六糖的诱导作用明显大于立枯病菌。这些结果表明,葡聚六糖可提高植株体内病情相关蛋白几丁质酶和β–1,3–葡聚糖酶的活性,通过较高水平的内源激素乙烯、IAA和ABA的代谢传递抗病信号,诱导菜薹植株对立枯病的系统抗性。

关键词: 菜薹, 葡聚六糖, 立枯病, 病程相关蛋白, 内源激素

Abstract: The influences of glucohexaose on sheath blight(Rhizoctonia solani)occurrence,pathogenesis-related proteins(PRs)and endogenous hormones contents were studied in flowering Chinese cabbage(Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)using the inoculation of R. solani at different glucohexaose levels. The results showed that glucohexaose could increase the resistance of plants to R. solani,and reduce the incidence of sheath blight,but had no effect on the yield. After inoculation with R. solani,the yield of flowering Chinese cabbage was reduced significantly compared with control. Glucohexaose treatments could alleviate the inhibition of R. solani on plant growth,and reduce the loss of the yield caused with R. solani. Both exogenous glucohexaose and inoculation with R. solani increased the activities of chitinase and β-1,3-glucanase,ethylene production, and the contents of indole acetic acid(IAA)and abscisic acid(ABA). These data suggested the involvement of endogenous hormones in the regulation of pathogenesis process of R. solani. It is important for inducing systematic acquired resistance of flowering Chinese cabbage to sheath blight to keep high level of PRs and endogenous hormones.

Key words: flowering Chinese cabbage, glucohexaose, sheath blight, pathogenesis-related protein, endogenous hormone

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