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园艺学报 ›› 2013, Vol. 40 ›› Issue (9): 1710-1718.

• 蔬菜 • 上一篇    下一篇

FISH 技术在芸薹属作物基因组研究中的应用进展

轩淑欣1,冯大领2,李岩宾1,赵玉靖1,张成合1,申书兴1,*   

  1. 1 河北农业大学园艺学院,河北保定071000;2 河北农业大学生命科学学院,河北保定071000
  • 出版日期:2013-09-25 发布日期:2013-09-25
  • 基金资助:

    国家‘863’课题(2012AA100202);国家自然科学基金项目(31171964);国家教育部高校博士点基金项目(20101302110001);河北省青年科学基金项目(C2010000738)

Recent Advances in Brassica Genome Research by Fluorescence in situ Hybridization(FISH)

XUAN Shu-xin1,FENG Da-ling2,LI Yan-bin1,ZHAO Yu-jing1,ZHANG Cheng-he1,and SHEN Shu-xing1,*   

  1. 1College of Horticulture,Agricultural University of Hebei,Baoding,Heibei 071000,China;2College of Life Science,Agricultural University of Hebei,Baoding,Heibei 071000,China
  • Online:2013-09-25 Published:2013-09-25

摘要: FISH 技术是进行基因和重复DNA 序列在染色体上可视化作图的精确有效方法,已广泛应用于特异核苷酸序列的物理作图、基因组或染色体的识别、DNA 序列的定量分析以及着丝粒、染色质空间结构分析等方面。综述了FISH 技术在芸薹属作物基因组研究中的重要进展,并讨论了其应用前景。

关键词: 芸薹属作物, 荧光原位杂交, 细胞遗传学, 染色体涂染, 伸展DNA 纤维

Abstract: Fluorescence in situ hybridization(FISH)is an effective and accurate method to visualize
the localization of genes and repetitive DNA sequences on chromosomes. This technique has been widely
used for physical mapping of unique nucleotide sequences on specific chromosome regions,for identifying
the specific genomes and chromosomes,for mearuring the size of various DNA molecules,and for
revealing the spatial organization of the centromere and heterochromatin or euchromatin of different
chromosomes. The present review mainly described the recent advances in Brassica genome research by
fluorescence in situ hybridization and discussed its future applications in Brassica crops.

Key words: Brassica crops, flourescence in situ hybridization(FISH), cytogenetics, genome, chromosome painting(CCP), extend DNA fibers(EDFs)

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