关键词: 韭菜, 根尖, 组织培养, 植株再生, 抗生素

Abstract: An efficient plant regeneration system was established through optimization of hormone combinat ion, genotype, seedling age and root induction medium in root tip culture of Chinese chive. Data showed that the best medium for callus and adventitious shoot differentiation wasMS medium supplemented with 1 mg/ L NAA and 2 mg/L BA. Shoot induction rate of 91-1, 91-8, Baoding Honggen, Shouguang Malinjiu and Lanzhou Xiaojiu were 78. 7%, 83. 7 % , 81. 9 %, 76. 7 % and 73. 3 %, while the average shoot number per explant were 40. 1 , 46. 7 , 36. 3 , 35. 4 and 44. 5 respect ively. Root t ips taken from plantlets 7 to 10 day old seedlings showed the highest regeneration ability. MS medium without growth regulators was optimum for root formation of regenerated shoots with 100% root induction rate and 14. 5 roots per shoot. Kanamycin inhibited callus and shoot induction definitely at 20 mg/ L. Carbenicillin and Cefotaxime had inhibit ing effects on callus induction and shoot regeneration at Carbenicillin 500 mg/ L and Ceftaxime 300 mg/ L respectively. The effect of Timent in was not obviously, with the inhibition effect only lying in the reduction of shoot number induced from callus.

Key words: Chinese chive ( Allium tuberosum Rottl. ex Spreng. ), Root tip, Tissue culture, Plant regeneration, Antibiotics