https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2003, Vol. 30 ›› Issue (03): 358-360.

• 研究报告 • 上一篇    下一篇

唐菖蒲组培脱毒技术研究

李昌禹;郭太君;焦培娟;张雅凤;杜令阁   

  1. (中国农业科学院特产研究所,吉林132109)
  • 收稿日期:2003-01-05 修回日期:2003-04-08 出版日期:2003-06-25 发布日期:2003-06-25

Studies on Virus-free and Rapid Reproduction in Cultural Plant of Gladiolus
and Its Effect

Li Changyu; Guo Taijun; Jiao Peijuan; Zhang Yafeng; Du Lingge
  

  1. (Institute of Special Economic Animal and Plant, Chinese Academy of Agricultural Sciences,Jilin 132109,China)
  • Received:2003-01-05 Revised:2003-04-08 Online:2003-06-25 Published:2003-06-25

摘要: 在MS培养基上,附加5 mg/L病毒唑培养唐菖蒲,再经38~40 ℃热处理,切取微茎尖两次,
去除了危害唐菖蒲的3种主要病毒TMV、CMV和TVY。以MS+ BA 0.5 mg/L+ NAA 0.5 mg/L+KT l mg/L为继代培养基,1/2MS无激素培养基为成球培养基,进行快繁获得了脱毒种球,移植后,植株生长发育状况得到明显改善。

关键词: 唐菖蒲, 脱毒

Abstract:

A study on tissue culture of gladiolus hybridus of ‘Chenghongjiao’ and ‘Ziyinghua’ was carried out in MS medium with diferent phyto-hormone BA,KT and NAA.A MS medium supplemented with BA 0.5 mg/L+NAA 0.5 mg/L+KT 1 mg/L was for the regenerated and 1/2MS medium without hormone was for bulbs forming.The three main viruses of TMV,CMV and TVY in gladiolus were removed from cultural plant of gladiolus in the regenerated medium through 5-20 mg/L concentration of ribavirin,treatment of high temperature 38-40℃
and 2 times of meristem-tip culture.

Key words:

G1adiolus, Virus-free, Effect

中图分类号: