Studies of Two Genes Related to Bacterial Wilt Resistance in Tomato

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (6): 1096-1104.

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Studies of Two Genes Related to Bacterial Wilt Resistance in Tomato

WANG Guo-ping1,*，CHU Meng2,*，KONG Jie2，CHEN Fu-ying2，CHEN Yu2，and ZHAO Ya-hua2,**

（1College of Horticulture，South China Agricultural University，Guangzhou 510642，China；2College of Life Sciences，South China Agricultural University，Guangzhou 510642，China）

Received:2014-01-23 Online:2014-06-25 Published:2014-06-25

Abstract

Abstract: Two-dimensional electrophoresis（2-DE）was employed to analyze the comparative proteome from stems of resistant tomato‘Hawaii 7996’plants inoculated with medium virulent strain RsM and high virulent strain RsH. Two differentially expressed proteins were identified to be Glutathione S-transferase L3 and Remorin 1 and the function of corresponding genes，GST-L3 and Rem-1，were further studied. After confirming the effectiveness of VIGS system with TRV carrying the Phytoene Desaturase gene（PDS），two targeted genes were individually silenced in Hawaii 7996 using VIGS method and their expression levels were checked by semi-quantitative RT-PCR. The results showed that the average disease incidence and disease index of bacterial wilt on GST-L3 or Rem-1 silenced plants increased significantly when challenging with RsM strain，indicating that these two genesare involved in the resistance of tomato to bacterial wilt disease.

Key words: tomato, bacterial wilt, resistance, two-dimensional electrophoresis, VIGS

CLC Number:

S 641.2

WANG Guo-ping1,*，CHU Meng2,*，KONG Jie2，CHEN Fu-ying2，CHEN Yu2，and ZHAO Ya-hua2,**. Studies of Two Genes Related to Bacterial Wilt Resistance in Tomato[J]. ACTA HORTICULTURAE SINICA, 2014, 41(6): 1096-1104.

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Studies of Two Genes Related to Bacterial Wilt Resistance in Tomato

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