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园艺学报 ›› 2014, Vol. 41 ›› Issue (1): 9-16.

• 果树 • 上一篇    下一篇

枳抗逆基因PtrZPT2-2 的克隆与表达分析

刘德春*,吴 启*,王玥辰,刘山蓓,刘 勇**   

  1. 江西农业大学农学院园艺系,南昌 330045
  • 出版日期:2014-01-25 发布日期:2014-01-25
  • 基金资助:

    国家自然科学基金项目(31260468);江西省自然科学基金项目(20114BAB214006);江西农业大学科学研究基金项目(CX201101)

Cloning and Expression Analysis of PtrZPT2-2 from Trifoliate Orange (Poncirus trifoliata

LIU De-chun*,WU Qi*,WANG Yue-chen,LIU Shan-bei,and LIU Yong**   

  1. Department of Horticulture,College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China
  • Online:2014-01-25 Published:2014-01-25

摘要: 通过电子克隆和RT-PCR 法从枳[Poncirus trifoliata(L.)Raf.]中克隆到1 个C2H2 型锌指蛋
白家族基因,命名为PtrZPT2-2。该基因编码159 个氨基酸残基,预测蛋白分子量为17.28 kD,等电点为
9.51。PtrZPT2-2 包含两个C2H2 型锌指结构域,在其C–末端含有一个转录抑制结构域EAR/DLN-box
(DLNL)。PtrZPT2-2 与其他植物参与各种非生物胁迫反应的C2H2 型锌指蛋白有较高的一致性。系统发
育树分析表明PtrZPT2-2 与杨树PtaZFP2,矮牵牛ZPT2-12、ZPT2-13,拟南芥ZAT12、ZAT7 亲缘关系较
近。半定量PCR 分析表明,低温、高盐、干旱胁迫和ABA 处理下枳叶、茎、根中PtrZPT2-2 均被诱导上
调。PtrZPT2-2 可能在枳适应低温、高盐、干旱等非生物胁迫和ABA 响应中起着重要作用。

关键词: 枳, C2H2 型锌指蛋白, PtrZPT2-2

Abstract: In the present study,a novel C2H2-type zinc finger protein gene,PtrZPT2-2,was cloned
from trifoliate orange by in silico and RT-PCR approaches. The PtrZPT2-2 encodes a protein of 159 amino
acid residues with a predicted molecular mass of 17.28 kD and an isoelectric point of 9.51. The PtrZPT2-2
protein contains two C2H2-type zinc finger domains,and a putative transcription repression domain
(EAR/DLN-box)at the C-terminus. Phylogenetic analysis showed that the PtrZPT2-2 clustered with
PtaZFP2,ZPT2-12,ZPT2-13,ZAT12 and ZAT7. PtrZPT2-2 shared high identity with other C2H2-type
zinc finger proteins involved in abiotic stress responses. Semi-quantitative PCR analysis showed that
expression of PtrZPT2-2 was upregulated by cold,salt,drought and ABA treatments in leaves,stems and
roots of trifoliate orange seedlings. Our data suggested that PtrZPT2-2 is a new member of the C2H2-type
zinc finger protein genes and may play an important role in response to cold,salt,drought and ABA
stresses in citrus.

Key words: trifoliate orange, C2H2-type zinc finger, PtrZPT2-2

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