关键词: 银杏, 细胞悬浮培养, 银杏内酯

Abstract: After determination of the content of ginkgolides from varieties of Ginkgo biloba L. , it was shown that therewere significantly different contents of ginkgolides from different genotypes of Ginkgo biloba L. , in which the highest were in Yuanling and Jinzhui, the contents reached 0. 43% and 0. 41% respectively, the lowest was in Qixingguo, the content was 0. 13%, the highest content of ginkgolides in calli from variet ies of Ginkgo biloba L.
was in Jinzhui and reached 0. 334 % . There were calli from 3 varieties producing no ginkgolide. The callus with high content of ginkgolides and growing well was cultured on B5 media. The effects of different growth factors on growth of cell and content of ginkgolides were studied. The result showed that 50 mL culture solution in the 150 mL flask, the inoculat ion 30- 40 g/L, the initial pH in culture solution 5. 8, 3 000- 4 000 lx lumination in the
intensity and the mixture of 30 g/ L sucrose and 15 g/L glucosewere optimum to synthesis of ginkgolides and growth of cell. The result by HPLC detection showed ginkgolides in suspension cells reached 0. 0785% of dry mass of cells
from the variety Yuanling.

Key words: Ginkgo biloba L., Cell suspension culture, Ginkgolides