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园艺学报 ›› 2003, Vol. 30 ›› Issue (02): 183-186.

• 研究论文 • 上一篇    下一篇

火炬姜离体快繁技术研究

潘学峰;王昌茂
  

  1. (海南大学农学院,海口 570228)
  • 收稿日期:2002-06-05 修回日期:2002-08-06 出版日期:2003-04-25 发布日期:2003-04-25

Studies On Rapid in Vitro Propagation of Phaeomeria magnisqca

Pan Xuefeng; Wang Cha
  

  1. (Agriculturae College,Hainan,Haikou 570228,China)
  • Received:2002-06-05 Revised:2002-08-06 Online:2003-04-25 Published:2003-04-25

摘要: 试验表明:火炬姜组培快繁以Ms+6-BA 2.0mg/L+NAA 0.1 mg/L+CW 10%作为增殖培养基可以获得理想的效果,增殖倍数可达4.3倍;以双芽为外植体在该培养基上经30 d培养可增殖5.4倍;在不含任何激素的1/2 Ms+0.15%活性炭培养基上即可形成较发达的根系,生根率达100%;试管苗移栽对基质要求不严,移栽到疏松透气、富含有机质的表土中,成活率高达100%,而且植株生长良好。

关键词: 火炬姜, 丛生芽, 组培快繁

Abstract: The research result of the in vitro propagation of the Phaeomeria magnifica is presented in this paper.The results showed that: Using MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+CW 10% ills proliferation median3. An optimal proliferation rate Wills obtained,the proliferation rate could reach 4.3 times:Using double shoots ills explants,after 30 clays of culture in this median3, it could amplify 5.4 times. The root formation of Phaeomeria magnifica tissue culture shoots had a low demand for exotic hormones,even in a medium of no hormones it could still form a gooa root system, the rooting rate could reach 100%.The transplantation of Phaeomeria magnisqca tissue culture shoots wills simple and feasible.There wills no strict demand for matrix,when the planflets were transplanted to superficial soil,the survival rate could reach 100%.and the plants grew well.

Key words: Phaeomeria magnisqca, Adventitious shoot, Rapid propagation via tissue culture

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