园艺学报 ›› 2008, Vol. 35 ›› Issue (2): 295-300.

• 研究简报 • 上一篇    下一篇



  1. (1 国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京 100102;2 中国林业科学研究院林业研究所,北京 100091)
  • 收稿日期:2007-07-16 修回日期:2007-09-20 出版日期:2008-02-25 发布日期:2008-02-25

Cloning and Sequence Analysis of a Flowering-related MADS-box Gene in Narcissus tazetta var. chinensis

GAO Zhi-min1, CHEN Duan-fen1, LI Xue-ping1, CAI Chunju1 and PENG Zhen-hua*2   

  1. (1 International Center for Bamboo and Rattan, SFA key Laboratory of Bamboo and Rattan Science and Technology, Beijing 100102, China;2 Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China)
  • Received:2007-07-16 Revised:2007-09-20 Online:2008-02-25 Published:2008-02-25

摘要: 采用RT-PCR技术从中国水仙(Narcissus tazetta var.chinensis)幼嫩花蕾中分离到一个新的水仙花发育相关MADS-box基因,命名为NTMADS1(GenBank登记号:EF421828)。该基因长879 bp,编码区共编码230个氨基酸,具有典型的植物MADS-box基因结构,由MADS区、I区、K区和C末端组成。序列分析结果表明,NTMADS1编码的蛋白与其它植物的MADS-box蛋白有着较高的一致性,其中与扫状天门冬(Asparagus virgatus)的AVAG1的一致性高达89%。系统进化树分析表明NTMADS1属于AG亚族。

关键词: 中国水仙, MADS-box 基因, 系统进化树分析


MADS-box genes play important role in various plant development processes,especially in the development of floral organs.The flowering-related MADS-box gene, named as NTMADS1 (GenBank accession number:EF421828), was cloned from the first strand of N. tazetta var. chinensis cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region. The fragment was 879 bp long and contained an open reading frame of 693 bp coding a polypeptide of 230 amino acids which had a typical MADS-box domain. Homology analysis showed that the deduced NTMADS1 protein was highly homologous to other MADS-box proteins from different species, especially with Asparagus virgatus to 89%. Phylogenetic tree analysis also indicated that NTMADS1 belongs to AG subfamily.

Key words: Narcissus tazetta var. chinensis, MADS-box gene, Phylogenetic tree analysis