植物生长调节剂对线艺春兰根状茎的增殖与分化的影响

园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 887-890.

植物生长调节剂对线艺春兰根状茎的增殖与分化的影响

石乐娟;张放^*;张士良;卜岩枫;叶晓伟

(浙江大学园艺系, 浙江杭州310029)

收稿日期:2005-10-08 修回日期:2005-12-12 出版日期:2006-08-25 发布日期:2006-08-25

Effects of Plant Growth Regulators on the Rhizomeps Multiplication and Differentiation in Cymbidium goeringii with Verge Line Pattern Leaves

Shi Lejuan;Zhang Fang;Zhang Shiliang; Bu Yanfeng;Ye Xiaowei

(Department of Horticulture, Zhejing Universty, Hangzhou, Zhejiang 310029, China)

Received:2005-10-08 Revised:2005-12-12 Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要： 以线艺春兰‘绿云’的类原球茎为材料, 研究了植物生长调节剂等因素对根状茎的增殖与分化的影响。结果表明: 长期继代培养及保存宜选用1 /2MS固体培养基。细胞分裂素6-BA浓度为1.0 mg·L ^{- 1}时根状茎的增殖效果最佳。0～3.0 mg·L ^{- 1}范围内IBA促进根状茎的生长较NAA效果好。1/2MS + 6-BA 1.0 mg·L ^{- 1} + IBA 1.0 mg·L ^{- 1}培养基最利于根状茎的分化, 芽分化率达到36.9%。培养基添加芋艿糊可显著促进根状茎的增殖与生长。根状茎分割以掰开方式且接种团块带有3～5条根状茎较适宜。线艺春兰叶片上的腹轮艺通过组培可以稳定遗传。

关键词: 春兰, 类原球茎, 根状茎, 6-BA, IBA

Abstract: The rapid multiplication system of Cymbidium goeringii‘Lüyun’with verge line pattern leaves by protocorm like-body was established. Effects of plant growth regulators on multiplication and differentiation of rhizomes were studied. 1 /2MS solid medium was suitable for subculture and preservation of the rhizome. The optimal medium for multiplication and growth of rhizomes was MS + 6-BA 1.0 mg·L^{- 1}. IBA was more effective to the growth of rhizomes than NAA with increasing of auxin concentration (0 - 3 mg·L ^{- 1} ).The optimum medium for differentiation of rhizomes was 1/2 MS supplemented with 6-BA 1.0 mg·L ^{- 1} and IBA 1.0 mg·L ^{- 1} , which regeneration rate of shoots was as high as 36.9%. Dasheen paste was better to the multiplication of rhizomes than coconut milk and banana mud added to the culture medium. Single conglomeration with 3 - 5 rhizomes was optimum by the way of broking off with fingers and thumb. Verge line pattern on the leaf could be stably inherited.

Key words: Cymbidium goeringii, Verge line pattern, Protocorm like-body, Rhizome, 6-BA, IBA

石乐娟;张放;张士良;卜岩枫;叶晓伟. 植物生长调节剂对线艺春兰根状茎的增殖与分化的影响[J]. 园艺学报, 2006, 33(4): 887-890.

Shi Lejuan;Zhang Fang;Zhang Shiliang;Bu Yanfeng;Ye Xiaowei. Effects of Plant Growth Regulators on the Rhizomeps Multiplication and Differentiation in Cymbidium goeringii with Verge Line Pattern Leaves[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 887-890.

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