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园艺学报 ›› 2016, Vol. 43 ›› Issue (5): 935-946.doi: 10.16420/j.issn.0513-353x.2016-0140

• 蔬菜 • 上一篇    下一篇

紫背天葵高通量转录组测序分析

张少平1,2,洪建基1,邱珊莲1,*,郑云云1,2,张 帅1,吴松海1,何炎森1,郑开斌1,2,郑加协1   

  1. (1福建省农业科学院亚热带农业研究所,福建漳州 363005;2福建省农业科学院作物研究所,福州350013)
  • 出版日期:2016-05-25 发布日期:2016-05-25

Sequencing and Analysis of the Transcriptome of Gynura bicolor

ZHANG Shao-ping1,2,HONG Jian-ji1,QIU Shan-lian1,*,ZHENG Yun-yun1,2,ZHANG Shuai1,WU Song-hai1,HE Yan-sen1,ZHENG Kai-bin1,2,and ZHENG Jia-xie1   

  1. (1Subtropical Agriculture Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China;2Crop Sciences Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)
  • Online:2016-05-25 Published:2016-05-25

摘要:

Gynura bicolor is rich in anthocyanidin and other functional components. In order to explore genetic basis for the synthesis of these active substancesthe transcriptome of Gynura bicolor was sequenced by Illumina HiSeq 2500 platforma total of 21 387 624 reading fragments were generated which formed 33 314 unigenes by sequence splicing. 2 387 SSR were found in 7 792 unigenes over 1 kb. All unigenes were annotated by different databases. 22 048 and 14 417 unigenes were annotated against Nr and SwissProt database respectivelyand 29 unigenes were related to anthocyanidin biosynthesisby Pfam database the 13 909 unigenes were included in 5 198 families of protein functional regions and 12 unigenes were related to anthocyanidin biosynthesisby GO database the 11 613 unigenes were divided into 3 categories containing 51 function groups and 24 unigenes were related to anthocyanidin biosynthesis;by COG and KOG databases the 6 589 and 13 498 unigenes were grouped into 24 functional categories and 25 ones,respectively;by KEGG database the 4 466 unigenes were divided into 108 metabolism pathways,47 unigenes involved flavonoid biosynthesis.

关键词: Gynura bicolor, transcriptome, sequencing, gene analysis, function annotation

Abstract:

Gynura bicolor is rich in anthocyanidin and other functional components. In order to explore genetic basis for the synthesis of these active substancesthe transcriptome of Gynura bicolor was sequenced by Illumina HiSeq 2500 platforma total of 21 387 624 reading fragments were generated which formed 33 314 unigenes by sequence splicing. 2 387 SSR were found in 7 792 unigenes over 1 kb. All unigenes were annotated by different databases. 22 048 and 14 417 unigenes were annotated against Nr and SwissProt database respectivelyand 29 unigenes were related to anthocyanidin biosynthesisby Pfam database the 13 909 unigenes were included in 5 198 families of protein functional regions and 12 unigenes were related to anthocyanidin biosynthesisby GO database the 11 613 unigenes were divided into 3 categories containing 51 function groups and 24 unigenes were related to anthocyanidin biosynthesis;by COG and KOG databases the 6 589 and 13 498 unigenes were grouped into 24 functional categories and 25 ones,respectively;by KEGG database the 4 466 unigenes were divided into 108 metabolism pathways,47 unigenes involved flavonoid biosynthesis.

Key words: Gynura bicolor, transcriptome, sequencing, gene analysis, function annotation

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