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园艺学报 ›› 2016, Vol. 43 ›› Issue (5): 918-926.doi: 10.16420/j.issn.0513-353x.2015-0929

• 蔬菜 • 上一篇    下一篇

红色和橙黄色果肉西瓜番茄红素和β–胡萝卜素代谢基因的表达分析

王 楠,刘 识,朱子成,王学征,栾非时*   

  1. (东北农业大学园艺学院,农业部东北地区园艺作物生物学与种质创制重点实验室,哈尔滨 150030)
  • 出版日期:2016-05-25 发布日期:2016-05-25

Expression Analysis of Lycopene and β-carotene Related Genes in Red and Orange-yellow Fleshed Watermelon Fruits

WANG Nan,LIU Shi,ZHU Zi-cheng,WANG Xue-zheng,and LUAN Fei-shi*   

  1. (Key Laboratory of Biology and Genetic Improvement of Horticulture Crops Northeast Region Ministry of Agriculture,Horticulture College,Northeast Agricultural University,Harbin 150030,China)
  • Online:2016-05-25 Published:2016-05-25

摘要:

以橙黄色果肉西瓜‘WM-Clr-2’及红色果肉西瓜‘PI 179881’为试材,采用HPLC法分析其番茄红素及β胡萝卜素7个发育时期的积累情况同时采用qRT-PCR技术分析番茄红素和β胡萝卜素代谢途径中8个关键酶基因PSY1PSY2PDSZDSCRTISOLCYBNCED1NCED7)的表达量。结果显示,随着果实的发育,番茄红素和β胡萝卜素含量均不断增加,且在同一时期,红色果肉‘PI 179881’中的含量始终大于‘WM-Clr-2’。qRT-PCR结果显示PSY1在‘PI 179881’中的表达量高于‘WM-Clr-2’,LCYBNCED1在‘WM-Clr-2’中的表达量高于红色果肉‘PI 179881’。分析番茄红素及β胡萝卜素的积累规律与相关基因表达规律说明PSY1的大幅上调表达,LCYB的下调表达及NCED1的低表达可能是红色果肉‘PI 179881’中番茄红素和β胡萝卜素大量积累的主要原因。PSY1的低表达水平,LCYBNCED1的高表达水平可能是橙黄色果肉‘WM-Clr-2’中番茄红素及β胡萝卜素积累量少的主要原因

关键词: 西瓜, 番茄红素, β–胡萝卜素, 果实发育, 基因表达

Abstract:

Two different fleshed watermelon cultivarsorange-yellow fleshedWM-Clr-2and red fleshedPI 179881were used in this study. Lycopene and β-carotene accumulation were analysised using HPLC at 7 growth stages through the whole fruit-set period for totally 56 days. The relative expression of eight genesPSY1PSY2PDSZDSCRTISOLCYBNCED1NCED7involved in the lycopene and β-carotene biosynthesis and biodegradation were analysised by qRT-PCR. According to the resultsthe accumulation of both lycopene and β-carotene were higher inPI 179881than inWM-Clr-2at each growth stage based on the phenotype of HPLC analysis. PSY1 gene were higher expressed inPI 179881than inWM-Clr-2on the contraryNCED1 and LCYB genes showed lower expression levels inPI 179881than inWM-Clr-2.The analysis of lycopene and β-carotene accumulation and the transcriptional expression of eight genes confirmed that a large quality of lycopene accumulated in red-fleshedPI 179881reflecting the elevated expression of the PSY1 genethe down-regulation of the LCYB gene and the low transcriptional levels of the NCED1 gene. The reduced transcriptional expression of PSY1 gene and the increased expression of NCED1 gene and LCYB gene were the main factors contributing to low accumulation of lycopene and β-carotene inWM-Clr-2.

Key words: watermelon, lycopene, β-carotene, fruit development, gene expression

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