消毒方式、无机盐浓度及光照强度对槲蕨孢子繁殖的影响

园艺学报 ›› 2009, Vol. 36 ›› Issue (5): 711-716.

消毒方式、无机盐浓度及光照强度对槲蕨孢子繁殖的影响

张银丽^1,2;杜红红^1,3;李　杨¹;李　东¹;季梦成²;姜闯道¹;石　雷^1*

(¹中国科学院植物研究所, 北京100093; ²浙江林学院园林学院, 浙江临安311300; ³东北林业大学生命科学学院,哈尔滨150040)

收稿日期:2008-10-20 修回日期:2009-04-13 出版日期:2009-05-25 发布日期:2009-05-25
通讯作者: 石　雷

Studies on the Effect of Different Sterilization Procedures , Medium,andDifferent Light Intensity on Spore Propagation of Drynaria roosii

ZHANG Yin-li^1,2,DU Hong-hong^1,3,L I Yang¹,LI Dong¹,JI Meng-cheng², JIANG Chuang-dao¹, and SHI Lei^1*

(¹Institute of B otany, the Chinese Academy of Sciences, Beijing 100093, China; ²The College of Landscape Architecture, Zhejiang Forestry University, Linpan, Zhejiang 311300, China; ³ The College of Life Science, Northeast Forestry University, Harbin150040, China)

Received:2008-10-20 Revised:2009-04-13 Online:2009-05-25 Published:2009-05-25
Contact: SHI Lei

摘要/Abstract

摘要：

研究了槲蕨（Drynaria roosii）孢子的无菌培养和常规繁殖方式，观察记录消毒方式、无机盐浓度和光照强度对槲蕨孢子萌发和配子体发育的影响.结果表明：槲蕨孢子接种后6～7 d即能萌发，60 d左右发育形成心形原叶体，100 d左右开始形成孢子体幼苗；采用NaClO消毒5.5 min左右是理想的消毒方式；在Knop’s培养基中孢子的萌发率最高，可达49%左右.低无机盐浓度的MS培养基有利于孢子萌发，但不利于配子体发育；黑暗条件下孢子能萌发但不能形成片状体.采用5% NaClO表面灭菌5.5 min， 1/2MS培养基在40 μmol·m^-2·s^-1光照条件下孢子萌发和配子体发育良好。

关键词: 槲蕨, 孢子萌发, 无菌培养

Abstract: The spore in vitro culture and routine propagation of Drynaria roosii were studied respectively. Tests were conducted to evaluate the effects of various sterilization procedures, medium and light intensity on spores of Drynaria roosii. The result exhibits that the spores germinated within 6-7 days after inoculated in the medium, and sporophytes began to form in 100 days after inoculated. The maximum germination ratio was 49% obtained in Knop’s medium. The low salt concentration of MS basal medium was helpful to spore germination, but inhibited the development of gametophytes. Spores can germinate in dark but can’t develop to prothallial plate. Spores surface sterilized for 5.5 min with 0.5%NaClO and inoculated in 1/2MS medium, were appropriate for Drynaria roosii cultivation with 40μmol·m^-2·s^-1 light intensity.

Key words: Drynaria roosii Nakaike, spore germination, in vitro culture

中图分类号:

S 68

张银丽;杜红红;李　杨;李　东;季梦成;姜闯道;石　雷. 消毒方式、无机盐浓度及光照强度对槲蕨孢子繁殖的影响[J]. 园艺学报, 2009, 36(5): 711-716.

ZHANG Yin-li;DU Hong-hong;L I Yang;LI Dong;JI Meng-cheng;JIANG Chuang-dao;and SHI Lei. Studies on the Effect of Different Sterilization Procedures , Medium,andDifferent Light Intensity on Spore Propagation of Drynaria roosii [J]. ACTA HORTICULTURAE SINICA, 2009, 36(5): 711-716.

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