关键词: 菊花, 春化作用, FLC, RT-PCR

Abstract: The full-length cDNA sequence of Chrysanthemum morflorium（Ramat.）Kitam.‘Yuuka’CmFLC-like1 gene was obtained using PCR and RACE. The cDNA was 945 bp in length with a 636 bp open reading frame which encodes a peptide of 211 residues. CmFLC-like1 has a high homology to VvFLC（Vitis vinifera）and DlFLC（Dimocarpus longan）contained a typical MADS domain，with the homology of 55% and 52%，respectively. CmFLC-like1 was located in the nuclear genome by subcellular localization. And it has no transcription activation activity in yeast system，while it show inhibitory activity in the plastid transformation system. CmFLC-like1 has the highest expression levels in leaves，while the lowest expression in stems and shoot tip，followed by root. The expression of CmFLC-like1 was inhibited by low temperature，especially at 4 ℃. Furthermore，the longer the cold treatment time，the more suppresser ofCmFLC-like1 expression.

Key words: chrysanthemum, vernalization, FLC, RT-PCR