Abstract: Three genes，respectively named SmTTG1，SmGL3 and SmTT8 were isolated from eggplant（Solanum melongena L.）using homology-based cloning method. Sequence analysis shows that the open reading frame（ORF）of SmTTG1 is 1 029 bp long，encoding 342 amino acids and sequence alignment reveals 94% identity with TTG1 in potato（S. tuberosum L.）. SmTTG1 protein contains four typical WD domains and protein isoelectric point is at pI 4.90. The ORF of SmGL3 is 1 887 bp long，encoding 628 amino acids and sequence alignmentreveals 94% identity with GL3 in potato. SmGL3 protein contains typical HLH domain and protein isoelectric point is at pI 5.61. While the ORF of SmTT8 gene is 1 896 bp long，encoding 631 amino acids and the multiple alignment of SmTT8 gene sequence with those of otherhomologues showed that it had high identity with homologous genein potato. SmTT8 protein contains typical HLH domain and protein isoelectric point is at pI 5.18. QPCR analysis indicates that SmTTG1，SmGL3 and SmTT8 are expressed in all organs of eggplants，including roots，stems，leaves，petals，peels and sarcocarp. However，the expression level of SmTTG1，SmGL3 and SmTT8 are tissue-specific. Yeast two-hybrid shows that SmTTG1 can interact with SmGL3 and SmTT8. Meanwhile，SmTTG1，SmGL3 and SmTT8 can interact with SmMYB. Therefore，it is speculated that SmTTG1 is a WD40 transcription factor and SmGL3 and SmTT8 are bHLH transcription factors，which are involved in the biosynthesis of anthocyaninin eggplant.

Key words: eggplant, anthocyanin, SmTTG1, SmGL3, SmTT8, yeast two-hybrid