甜瓜属人工异源四倍体早期基因组变化的初步研究

园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1105-1107.

甜瓜属人工异源四倍体早期基因组变化的初步研究

陈龙正;娄群峰;Joseph N. Wolukau;陈劲枫;耿　红;罗向东

(南京农业大学园艺学院, 作物遗传与种质创新国家重点实验室, 南京210095)

收稿日期:2004-12-24 修回日期:2005-03-30 出版日期:2005-12-25 发布日期:2005-12-25

Preliminary Studies on Early Genomic Changes of a Synthetic Allotetraploidin Cucumis

Chen Longzheng;Lou Qunfeng;Joseph N. Wolukau;Chen Jinfeng;Geng Hong;Luo Xiangdong

(College of Horticulture, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China)

Received:2004-12-24 Revised:2005-03-30 Online:2005-12-25 Published:2005-12-25

摘要/Abstract

摘要： 利用AFLP技术对甜瓜属人工异源四倍体(Cucumis ×hytivus Chen and Kirkbride, 2n = 38) 不同自交世代及二倍体亲本DNA进行分析, 结果表明多倍体基因组在形成早期发生了广泛的变化, 变化位点所占比例高达22.2%。基因组序列变化始于F₁ 代, 最高变化率出现在S2 代。基因组变化主要表现为部分亲本片段丢失、新片段产生以及后代对亲本某些位点的跳跃式继承。还观察到部分双亲差异性条带呈现出偏父性遗传的特点。

关键词: 甜瓜属, 异源四倍体, AFLP分析, 基因组序列变异

Abstract: AFLP analysis was carried out in amplification of the DNAs from different generations of the synthetic allotetraploid (Cucumis ×hytivus, Chen and Kirkbride, 2n = 38) , the interspecific F1 , and its diploid parents. The results indicated that extensive genomic changes occurred in the synthetic allotetraploid, and the variation rate was as high as 22.2%. We found the genomic changes started in the F₁ and the highest variation rate observed in S₂. Most genomic changes involved the loss of parental fragments, formation of novel fragments, with some fragments disappearing in one generation and reappearing in others. In addition, it was observed that some parent-specific bands were inherited preferentially from the male parent.

Key words: Cucumis, Allotetraploid, AFLP, Genomic change

中图分类号:

S 652

陈龙正;娄群峰;Joseph N. Wolukau;陈劲枫;耿　红;罗向东. 甜瓜属人工异源四倍体早期基因组变化的初步研究[J]. 园艺学报, 2005, 32(6): 1105-1107.

Chen Longzheng;Lou Qunfeng;Joseph N. Wolukau;Chen Jinfeng;Geng Hong;Luo Xiangdong. Preliminary Studies on Early Genomic Changes of a Synthetic Allotetraploidin Cucumis[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1105-1107.

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