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园艺学报 ›› 2007, Vol. 34 ›› Issue (5): 1327-1328.

• 新技术与新方法 • 上一篇    下一篇

珍稀濒危植物珙桐离体快繁技术初步研究

金晓玲1*; 吴安湘2; 沈守云1; 章怀云3   

  1. 1 中南林业科技大学环境艺术设计学院, 长沙 410004; 2 桂林旅游高等专科学校,广西桂林 541004;3中南林业科技大学生命科学学院, 长沙 410004)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

A Preliminary Study on thein Vitro Culture of Endangered Plant Davidia involucrata Baill.

JIN Xiao-ling1*; WU An-xiang2; SHEN Shou-yun1; ZHANG Huai-yun3   

  1. (1 Environment and Art Design School, Central South University of Forestry and Technology, Changsha 410004, China; 2 Guilin Institute of Tourism, Guilin, Guangxi 541004, China; 3College of Life Science, Central South University of Forestry and Technology, Changsha 410004, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要: 研究了我国一级珍稀濒危树种珙桐(Davidia involucrata Baill.) 带芽茎段的离体培养。筛选出最佳培养基: ( 1) 冬芽诱导培养基: WPM+BA 2.0 mg·L-1+NAA 0.1 mg·L-1; (2) 丛生芽诱导培养基: WPM + BA 2.0 mg·L-1; (3) 丛生芽增殖培养基: WPM+ BA 2.0 mg·L-1+ ZT 0.1 mg·L-1; (4) 生根培养基: WPM + NAA 0.5 mg·L-1

关键词: 珙桐, 茎段, 丛生芽, 离体培养

Abstract: Davidia involucrata Baill. named Chinese dove tree. It is not only a fomous ornamental plant but also a rare and endangered plant. This paper mainly dealt with the study on shoot organogenesis culture in vitro culture from dormant bud explants of Davidia involucrata. The results showed that the best media for various stages were as follows:(1) The dormant bud induction medium: WPM+BA 2.0 mg·L-1+NAA 0.1 mg·L-1; (2) Clump shoot induction medium: WPM + 2.0 mg·L-1 BA;(3) Clump shoot regeneration medium: WPM+ BA 2.0 mg·L-1+ ZT 0.1 mg·L-1;(4) Rooting medium: WPM + NAA 0.5 mg·L-1.

Key words: Davidia involucrata Baill., Shoot stem, Clump shoot, In vitro culture