基于Adh 基因家族序列的牡丹组( Sect. Moutan DC. ) 种间关系

园艺学报 ›› 2004, Vol. 31 ›› Issue (5): 627-632.

基于Adh 基因家族序列的牡丹组( Sect. Moutan DC. ) 种间关系

林启冰^1,2;周志钦^1,2*;赵宣^1,2;潘开玉¹;洪德元^1*

(¹ 中国科学院植物研究所系统与进化植物学重点实验室, 北京100093 ; ²西南农业大学园艺系, 重庆400716)

收稿日期:2003-12-15 修回日期:2004-04-02 出版日期:2004-10-25 发布日期:2004-10-25
通讯作者: 周志钦;洪德元

Interspecif ic Relationships among the Wild Species of Paeonia Sect. MoutanDC. Based on DNA Sequences of Adh Gene Family

Lin Qibing^1,2;Zhou Zhiqin^1,2*;Zhao Xuan^1,2;Pan Kaiyu¹;Hong Deyuan^1*

(¹ Laboratory of Systematic and Evolutionary Botany , Institute of Botany , Chinese Academy of Sciences , Beijing 100093 ,China ; ² Department of Horticulture , Southwest Agriculture University , Chongqing 400716 , China)

Received:2003-12-15 Revised:2004-04-02 Online:2004-10-25 Published:2004-10-25
Contact: Zhou Zhiqin1;Hong Deyuan

摘要/Abstract

摘要： 测定了牡丹组7 个种10 个野生居群共10 份样品的全部Adh1A、Adh1B 和Adh2 基因序列。结合前人已报道的牡丹野生种的A dh 基因序列, 以芍药组(Sect . Paeonia) 和北美芍药组(Sect . Oneapia)的种作外类群, 用PAUP 3 (4.0 b 4 a) 计算机程序构建了牡丹组全部8 个种的A dh1A、A dh1B 和A dh2基因树(最大简约树和邻接树) , 同时进行了Adh1A、Adh1B 和Adh2 基因序列的合并分析。结果表明,牡丹组的8 个种分为两个具有90%以上自展值支持的单系分支, 分别对应Stern (1946) 根据形态划分的革质花盘亚组(Subsect . V aginatae) 和肉质花盘亚组(Subsect . Delavayanae) 。在革质花盘亚组内, 本研究结果支持银屏牡丹( Paeonia suffruticosa ssp. yinpingmudan) 和凤丹( P. ostii ) 、紫斑牡丹( P. rockii ) 和四川牡丹( P. decomposita) 以及矮牡丹( P. jishanensis) 和卵叶牡丹( P. qiui ) 各种之间有更近的亲缘关系, 但有关牡丹复合体( P. suffruticosa complex) 内各种间更进一步的关系还有待进一步研究。根据Adh 基因树并结合前人的结果对牡丹组的种间关系进行了详细的讨论。

关键词: 芍药属, 牡丹组, Adh 基因, DNA 序列, 种间关系

Abstract: Five out of eight species in the Paeonia Sect . Moutan were suggested to be the wild ancestors of the cultivated tree peonies in China. Therefore , it is of great importance both in theory and practice to have a better understanding of the relationships among the species of Paeonia Sect . Moutan DC. . In this study , a portion (five exons and four introns) of the alcohol dehydrogenase (ADH) genes including Adh1A , Adh1B , and Adh2 of 10 accessions of wild tree peonies collected from ten wild populations representing 7 species of
Paeonia Section Moutan were sequenced and analyzed. Using species in the Section Paeonia and Section Oneapia as outgroups , both maximum parsimony (MP) and neighbor-joining (NJ ) trees of all 8 wild species
of Paeonia Section Moutan for each gene were const ructed with PAUP 3 (version 4.0 b 4 a) computer program based on the sequences obtained in this study and those downloaded from the Genbank. A combined analysis of Adh1A , Adh1B and Adh2 gene sequences was also carried out . Results obtained in this study indicate that eight species of Paeonia Section Moutan form two st rongly supported (bootstrap > 90 %) monophyletic clades which respond to Subsect . V aginatae and Subsect . Delavayanae of Sternps system (1946) . Within the Subsect . Vaginatae , our results support that there are close relationships between P. suffruitcosa ssp. yinpingmudan and P. ostii , P. decomposita and P. rockii , and P. jishanensis and P. qiui . However , for the general interspecific relationships among the species in P. suffuticosa complex , new pieces of evidence are still needed. Based on the gene t rees obtained in this study and results reported in previous literature , theinterspecific relationship of Paeonia Section Moutan was discussed in details

Key words: Paeonia, Section Moutan, Adh genes, DNA sequences, Interspecific relationship

中图分类号:

S 68

林启冰;周志钦;赵宣;潘开玉;洪德元. 基于Adh 基因家族序列的牡丹组( Sect. Moutan DC. ) 种间关系[J]. 园艺学报, 2004, 31(5): 627-632.

Lin Qibing;Zhou Zhiqin;Zhao Xuan;Pan Kaiyu;Hong Deyuan. Interspecif ic Relationships among the Wild Species of Paeonia Sect. MoutanDC. Based on DNA Sequences of Adh Gene Family[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 627-632.

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