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园艺学报 ›› 2020, Vol. 47 ›› Issue (6): 1098-1110.doi: 10.16420/j.issn.0513-353x.2019-0813

• 研究论文 • 上一篇    下一篇

中国硬叶兜兰主要分布区居群表型多样性分析

程 浩1,徐玉凤2,王文晓2,朱 俊2,贾瑞冬1,*,杨树华1,赵 鑫1,葛 红1,*   

  1. 1中国农业科学院蔬菜花卉研究所,国家花卉改良中心,北京 100081;2河南农业大学林学院,郑州 450002
  • 出版日期:2020-06-25 发布日期:2020-06-25
  • 基金资助:
    国家自然科学基金项目(31301810);国家公益(农业)行业科研专项(201203071);农业部园艺作物生物学与种质创制重点实验室项目

Phenotypic Diversity of Paphiopedilum micranthum Populations from Main Distribution Areas in China

CHENG Hao1,XU Yufeng2,WANG Wenxiao2,ZHU Jun2,JIA Ruidong1,*,YANG Shuhua1,ZHAO Xin1,and GE Hong1,*   

  1. 1National Flowers Improvement Center of China,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2 College of Forestry,Henan Agricultural University,Zhengzhou 450002,China
  • Online:2020-06-25 Published:2020-06-25

摘要: 研究基于硬叶兜兰(Paphiopedilum micranthum)表型变异,通过相关性、主成分以及聚类等分析,对9个硬叶兜兰野生居群的22个表型性状指标进行了调查研究,探究硬叶兜兰居群间及居群内表型多样性,为硬叶兜兰资源的进一步保护和利用奠定基础。结果表明:硬叶兜兰在居群间具有丰富的表型多样性,居群内变异较少。平均表型分化系数(Vst)为64%,居群间表型分化高于居群内表型分化。22个性状的平均变异系数(CV)范围为9.52% ~ 31.48%,9个居群平均变异系数为14.55% ~ 18.51%,在表型间差异显著,在居群间差异不显著。主成分分析表明,花纵径等花部性状和株高等营养性状是硬叶兜兰变异的主要因素。Pearson相关性分析表明,株高、中萼片长和花瓣长与大部分性状都呈现显著正相关;冠幅、叶片数等营养性状与海拔存在显著正相关性,而花横径、花纵径等生殖性状与海拔存在显著或极显著负相关性。聚类分析结果表明,9个居群可以聚类为3个类群,和硬叶兜兰地理分布距离一致。

关键词: 硬叶兜兰, 居群, 表型多样性, 资源保护

Abstract: This study was designed to characterize the phenotypic diversity of Paphiopedilum micranthum,with the aim of creating a foundation for the further protection and use of Paphiopedilum germplasm resource. Analysis of 22 morphological characteristics among 9 natural populations was performed through statistical analysis methods including correlation,principal component and cluster analysis. The results showed that there were significant differences in most phenotypic traits among populations but few differences within populations. The mean phenotypic differentiation coefficient(Vst)was 64%,and the variation among populations was higher than that within populations. The average coefficient of variation(CV)was 9.52%–31.48% among 22 traits and 14.55%–18.51% among nine populations. The variation coefficients varied dramatically among phenotypic traits. Principal componentanalysis(PCA)revealed that traits including vertical diameter of flower and plant height were responsible for the diversity of P. micranthum. Pearson correlation analysis showed plant height,length of dorsal sepal and length of petal displayed significant positive correlation with the majority of traits. Vegetative characteristics,such as canopy diameter and leaf number,were positively correlated with altitude,whereas reproductive characteristics,such as diameter and vertical diameter of flower,were negatively correlated with altitude. According to the cluster analysis,the nine P. micranthum populations were divided into three groups,which is consistent with their geographic distributions.

Key words: Paphiopedilum micranthum, population, phenotypic diversity, resource conservation

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