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园艺学报 ›› 2020, Vol. 47 ›› Issue (4): 749-758.doi: 10.16420/j.issn.0513-353x.2019-0408

• 研究报告 • 上一篇    下一篇

菜豆金黄荚色产生的生化机制研究

李艳美1,刘大军2,*,冯国军2,刘 畅2,杨晓旭2,范有君2,闫志山2   

  1. 1黑龙江大学生命科学学院,哈尔滨150080;2黑龙江大学农作物研究院,哈尔滨 150080
  • 出版日期:2020-04-25 发布日期:2020-04-25
  • 基金资助:
    哈尔滨市高层次人才项目(2017RAGXJ003);黑龙江省自然科学基金联合引导项目(LH2019C058);黑龙江省应用技术研究与开发计划项目(GA19B102)

Biochemical Mechanism of Golden Yellow Pod Color in Common Bean

LI Yanmei1,LIU Dajun2,*,FENG Guojun2,LIU Chang2,YANG Xiaoxu2,FAN Youjun2,and YAN Zhishan2   

  1. 1College of Life Sciences,Heilongjiang University,Harbin 150080,China;2Crop Research Institute,Heilongjiang University,Harbin 150080,China
  • Online:2020-04-25 Published:2020-04-25

摘要: 选用菜豆金黄色豆荚品种‘A18’和绿色豆荚品种‘热那亚’为试验材料,通过测定豆荚发育过程中荚壁的叶绿素、类胡萝卜素和叶绿素合成途径中相关代谢物质含量,探讨菜豆金黄荚色的形成机理。结果表明,嫩荚呈现金黄色是由于叶绿素缺乏,推测是因为叶绿素酸酯a(chlorophyllide a)的合成受阻导致;利用荧光定量PCR对叶绿素生物合成相关基因的表达进行分析,发现在‘A18’中CHLM和DVR的表达量从花后12 d开始下降,但CHLP、CHLG和CAO的表达量在多个时间点高于‘热那亚’,这说明虽然叶绿素酸酯a合成受阻,但叶绿素的合成仍然能进行。

关键词: 菜豆, 荚色, 光合色素, 叶绿素合成途径, 叶绿素合成相关基因

Abstract: Golden yellow bean pod‘A18’and green bean‘Genoa’were chosen as experimental materials to study the expressing mechanism of color in golden bean pods. The formation mechanism of the golden pod color of kidney bean was studied by determining the amount of related metabolin in the synthetic pathway of chlorophyll,carotenoid,and chlorophyll during pod development in its wall. The results indicated the golden color of tender pod was resulted from the lack of chlorophyll content,which was speculated by the inhibition of chlorophyllide a synthesis. The expression pattern of chlorophyll biosynthesis-related genes was analyzed by real-time PCR. Indeed,the analysis suggested the expression levels of CHLM and DVR decreased in‘A18’from the 12th day after flowering. While the expression levels of CHLP,CHLG and CAO were higher than those in‘Genoa’at multiple time points,indicating that although chlorophyllide a synthesis is blocked,chlorophyll synthesis is still active.

Key words: Phaseolus vulgaris, pod color, photosynthetic pigment, chlorophyll synthesis pathway, chlorophyll synthesis related gene

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