%0 Journal Article %A ZHAO Kaixi %A DING Xi %A and WANG Yuejin* %T Research on Control of Disease Resistance by Stilbene Synthase Genes Derived from Chinese wild Vitis quinquangularis %D 2020 %R 10.16420/j.issn.0513-353x.2020-0263 %J Acta Horticulturae Sinica %P 1264-1276 %V 47 %N 7 %X Two complete open reading frames of VqSTS11 and VqSTS23,were respectively isolated from Vitis quinquangularis‘Danfeng-2’using homologous cloning technique and further for overexpression vector construction. Meristematic bulk(MB)of cultivated Vitis vinifera‘Thompson Seedless’,were induced through organogenesis and further for Agrobacterium-mediated transformation to obtain transgenic plants. After PCR detection and Western blot identification,5 transgenic lines of VqSTS11 and 3 transgenic lines of VqSTS23 were verified,respectively. Transgenic plants and non-transgenic plants were artificially inoculated with powdery mildew. Microscopic observation demonstrated that the hyphae growth in transgenic lines were slower than those in non-transgenic plants and spore germination was inhibited in transgenic plants. The disease resistance of transgenic plants was further analyzed by qRT-PCR and HPLC. The results displayed that not only the expression of STSs in transgenic plants increased,and the expression of disease resistance-related genes was up-regulated,but also stilbene compounds in the transgenic plants were accumulated. The expression product of‘Danfeng-2’VqSTS11 and VqSTS23 could tolerate powdery mildew. Therefore,‘Danfeng-2’could be used to improve the disease tolerance and provide disease- tolerant candidate genes for European grape cultivars,which could be served as a breeding resources for disease-tolerant breeding. %U https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0263