%0 Journal Article %A Rui ZHANG %A Xiayi ZHANG %A Ting ZHAO %A Shuangcheng WANG %A Zhongxing ZHANG %A Bo LIU %A De ZHANG %A Yanxiu WANG %T Transcriptome Analysis of the Molecular Mechanism of Saline-alkali Stress Response in Malus halliana Leaves %D 2022 %R 10.16420/j.issn.0513-353x.2021-0077 %J Acta Horticulturae Sinica %P 237-251 %V 49 %N 2 %X

Malus halliana is a highly saline-alkali-resistant apple rootstock in north-western China. The genes and main pathways which respond to saline-alkali stress were identified by RNA-Seq. This study revealed the mechanism of the response to salt damage and provided theoretical basis for the salt tolerance mechanism of apple rootstocks. Normal water(Hoagland nutrient solution)(Control)supply and mixed saline-alkali stress(Hoagland nutrient solution + 100 mmol · L-1 NaCl + NaHCO3)(Treatment)were set up to screen the saline-alkali resistance of each treatment in the growth period. These two samples before and after saline-alkali treatment were analyzed by RNA-seq. The function and pathway enrichment of differentially expressed genes(DEG)were also performed by GO and KEGG analysis. Twenty DEG were randomly selected for further qRT-PCR analysis to verify the RNA-seq data. A total of 16 246 DEGs were determined,7 268 genes were up-regulated and 8 978 genes down-regulated under saline-alkali stress. GO analysis fund that the DEGs were considerably differenct in the biological processes,cell components and molecular functions. KEGG analysis indicated that most enriched saline-alkali-responsive genes were mainly involved in signal transduction,carbon metabolism,biosynthesis of amino acids,and other secondary metabolites. DEG mainly focused on calcium signaling pathway,plant hormone signal transduction,phenylalanine metabolism,and carotenoid biosynthesis. qRT-PCR detection results of 20 DEG expression patterns were similar to those of RNA-seq. Among them,aspartate aminotransferase(GOT1),monodehydroascorbate reductase(NADH),chalcone isomerase(CHI,E5.5.1.6),β-carotene 3-hydroxylase(CrtZ)genes were highly expressed during saline-alkali stress. The results indicated that they might play a role in the leaf response to saline-alkali stress. M. halliana is mainly regulated by calcium signal pathway,plant hormone signal transduction,biosynthesis of amino acids, carotenoid biosynthesis and other secondary metabolites to respond to saline-alkali stress.

%U https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0077