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2019, Vol.46, No.8 Previous Issue    Next Issue

Research Papers

  • Expression Analysis of Apple MdCYP707A Family Genes and Functional Characterization of MdCYP707A1
  • ZHANG Tingting,KANG Hui,FU Lulu,YOU Chunxiang,WANG Xiaofei*,and HAO Yujin*
  • Acta Horticulturae Sinica. 2019, 46(8): 1429-1444. DOI:10.16420/j.issn.0513-353x.2018-0961
  • Abstract ( 358 ) HTML ( 799 ) PDF (3702KB) ( 799 )    
  • In this study,we analyzed the protein sequences and conserved motif of four apple MdCYP707A family members,and found that the four MdCYP707A family proteins contained a cytochrome P450 monooxygenase domain. The expression levels of the four genes in different tissues (roots,stems,leaves,flowers,fruits,and seeds)were detected by real-time qPCR,and they exhibited the highest level in the seeds. The expression pattern of MdCYP707As also showed significant difference during fruit developmental stages. We also examined the transcription level of the MdCYP707A family members in the process of water swelling and stratification of apple seeds,and found that the MdCYP707A family members were involved in the degradation of ABA during seed germination. Furthermore,the response of MdCYP707A genes to abiotic stresses(such as drought,salt,osmotic stress)and ABA treatments indicated their important roles in seed germination,among them,the response to ABA is most obvious. In addition,the function of MdCYP707A1 gene was identified through Agrobacterium-mediated genetic transformation to apple calli and Arabidopsis. The results demonstrated that overexpression of MdCYP707A1 reduced the resistance to abiotic stress,which suggests that it may be involved in ABA degradation,and ectopic expression of MdCYP707A1 in Arabidopsis increased the germination rate.

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  • Cloning,Expression Pattern and Promoter Activity Analysis of Flowering Regulatory Gene SVP in Apple(Malus × domestica)
  • WANG Shixiang,ZUO Xiya,XING Libo,FAN Sheng,ZHANG Dong,HAN Mingyu,and ZHANG Linsen*
  • Acta Horticulturae Sinica. 2019, 46(8): 1445-1457. DOI:10.16420/j.issn.0513-353x.2018-0918
  • Abstract ( 310 ) HTML ( 712 ) PDF (3430KB) ( 712 )    
  • The key gene MdMADS50 of SHORT VEGETATIVE PHASE(SVP)was cloned by homologous recombination method using short shoot buds of‘Nagafu 2’apple. The open reading frame (ORF)of MdMADS50 was 675 bp,that encoded 224 amino acids. Protein sequence analysis revealed that this gene contains conserved MADS-box and K-box domains belonging to MIKC-type MADS-box genes. Phylogenetic analysis indicated that the MdMADS50 protein has high homology with MdSVP. By quantitative real-time PCR(qRT-PCR)tissue-specificity expression of MdMADS50 was identified,that displayed that MdMADS50 has higher expression in shoots and leaves. In addition,exogenous GA3 treatment promoted the expression of MdMADS50,and the expression level of cultivar ‘Nagafu 2’in the initial stage of flower induction was significantly higher compared to other cultivars. Furthermore,the GUS activity assay showed that the MdMADS50 promoter had promoter activity and was enhanced by exogenous GA3. In conclusion,our study showed that MdMADS50 may significantly inhibit the flower induction of apple in response to GA3 treatment,mediating the regulation of gibberellin signaling in apple flower buds.
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  • Expression Pattern and Function in Juvenile Regulation of B-box Gene PuBBX24 in Pyrus ussuriensis
  • WANG Rihong,SONG Minyan,WANG Ran,and YANG Yingjie*
  • Acta Horticulturae Sinica. 2019, 46(8): 1458-1472. DOI:10.16420/j.issn.0513-353x.2018-1020
  • Abstract ( 232 ) HTML ( 479 ) PDF (3948KB) ( 479 )    
  • To understand the function of PuBBX24 in pear growth and development process,the gene of PuBBX24 and its promoter sequence were cloned from Pyrus ussuriensis seedling for molecular biological analysis. The spatial expression pattern of PuBBX24 was analyzed in roots,stems,leaves,flowers,and fruits. The cis-elements of PuBBX24 promoter were predicted and subsequently transferred into Arabidopsis with GUS gene for spatial-temporal,as well as under conditions of ABA,different light and other abiotic stresses analysis of its activity. The function of PuBBX24 was also heterologously analyzed in Arabidopsis. The results showed that PuBBX24 had different expression patterns in roots,stems,leaves,flowers,and fruits,with the highest expression level in stems and lowest expression level in leaves. The PuBBX24 expression level decreased gradually in the leaves from juvenile to adult period. A series of cis-elements of PuBBX24 promoter were found with annotations of hormone-responsive,light-responsive and stress-responsive elements. The expression of GUS in transgenic Arabidopsis was mainly detected in leaves,hypocotyls,and sepals,with no uniform levels in roots,lower level in stigma and pedicel. In addition,GUS showed a gradually decreased expression pattern in the rosette leaves from juvenile to adult period and undetectable GUS activity was found in silique. PuBBX24 promoter showed obvious response to stresses of ABA,light,low temperature,osmosis and salt treatments,and over-expression of PuBBX24 in Arabidopsis affected the juvenile-adult transition. These results indicated that PuBBX24 might be involved in responses to ABA,light,and other abiotic stresses and play an important role in regulating the juvenile-adult transition process.

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  • Grey Correlation Analysis of Physiological and Biochemical Factors in Embryo Abortion of Seedless Grape
  • JI Wei1,2,3,*,GUO Rongrong1,2,3,*,WANG Jingbo1,2,3,JIAO Xiaobo1,2,3,YAN Zhao4,CHANG Qinxiang4,DONG Zhigang2,5,and WANG Yuejin1,2,3,**
  • Acta Horticulturae Sinica. 2019, 46(8): 1473-1485. DOI:10.16420/j.issn.0513-353x.2019-0011
  • Abstract ( 232 ) HTML ( 463 ) PDF (2435KB) ( 463 )    
  • This study was to determine the major physiological and biochemical factors affecting embryo abortion in seedless grape. Two seedless grape varieties,‘Wuhe Cuibao’and‘Lihongbao’,and one seeded variety,‘Cabernet Sauvignon’(control),were used. Morphological index,physiological and biochemical change in pulp and ovule were observed and measured at various days after full bloom. Major parameters related to embryo abortion were determined using principal component analysis and grey correlation analysis. The results showed that the rate of ovule deformity reached the highest at 36 days after full bloom for‘Wuhe Cuibao’and at 32 days after full bloom for‘Lihongbao’,at those days the ovules began to abort. In the three grape varieties,the content of MDA in ovule and the content of soluble sugar and soluble protein in pulp increased with fruit development. In both seedless varieties,soluble sugar and protein in ovule reached the highest content during abortion,and then decreased. The activities of POD and CAT in seedless grapes first increased and then decreased. During abortion,ovule POD and CAT activities were significantly higher in the seedless varieties compared to the seeded variety,but the converse was also true for pulp POD and CAT activities. In the seedless varieties,the activity of SOD in ovule increased significantly before abortion; in the seeded variety,ovule SOD activity increased slowly with time. The analysis demonstrated that in seedless grape the major factors affecting embryo abortion are pulp soluble sugar and protein,ovule MDA,ovule soluble protein and ovule SOD. Among them,ovule SOD activity showed the best correlation with embryo abortion,followed by pulp soluble protein content and ovule number. This study could provide the theoretical guidance for determining sampling time in embryo-rescue breeding programs for seedless grape.

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  • Effect of CaCl2 Treatment on Cell Wall Degrading Enzymes Activities and Microstructure of Fruit Cracking of Ziziphus jujuba‘Huping Zao’
  • GUO Hongyan1,BAI Jinhua1,DUAN Fengqin1,XI Xin1,LI Tao1,and GUO Jinping2,*
  • Acta Horticulturae Sinica. 2019, 46(8): 1486-1494. DOI:10.16420/j.issn.0513-353x.2018-0832
  • Abstract ( 207 ) HTML ( 543 ) PDF (1933KB) ( 543 )    
  • Fruit cracking is seriously harmful to fruit production of Ziziphus jujuba in Northern China. The present study was undertaken to identify agronomic measures to reduce the dehiscent fruit rate of Ziziphus jujuba Mill.‘Huping Zao’,which is the most popular cultivar planted in Shanxi Province. The 0.01 g ? mL-1 CaCl2 solution was sprayed during the young fruit stage,fruit expanding stage,and fruit mature period respectively in the field. Then,the dehiscent fruit rate,fruit cell wall degrading enzymes,contents of cell wall compositions,and fruit microstructure were observed. The results showed the dehiscent fruit rate and activities of pectinase and cellulose were significantly decreased with the foliar application of calcium,while the contents of protopectin and cellulose were significantly improved in pericarp and pulp. The dehiscent fruit rate was positively correlated to the activities of pectinase and cellulose,but negatively correlated to the content of protopectin and cellulose. According to the microstructure,the cuticle of jujube fruit without crack was more intact and thicker than the control. Moreover,the fruit epidermal cells were more tightly arranged and smaller than the control,with less cavity in pulp cells. Our data suggested that foliar application of calcium could be applied as an important measure to prevent fruit cracking during both young fruit and fruit expanding stages.

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  • Genome-wide Association Studies for Monoecism in Spinach
  • WANG Haoying1,2,*,LIU Zhiyuan2,*,WANG Xiaowu2,WU Jian2,ZHANG Helong2,XIA Zhilan1,XU Zhaosheng2,**,and QIAN Wei2,**
  • Acta Horticulturae Sinica. 2019, 46(8): 1495-1502. DOI:10.16420/j.issn.0513-353x.2019-0081
  • Abstract ( 244 ) HTML ( 481 ) PDF (822KB) ( 481 )    
  • Spinach is a typical dioecious plant,but some varieties produce monoecious plant. In order to locate the genes controlling the monoecious traits of spinach,the sexual genotypes of 82 spinach inbred lines were investigated and analyzed. Each sample isolated from these accessions was re-sequenced with 10-fold sequencing depth,and a total of 3 420 496 SNPs were obtained. After filtration,1 621 970 high-quality polymorphic SNPs were acquired. The genome-wide association study(GWAS)of monoecious was performed by using 1 621 970 high-quality SNPs. The compress mixed linear model(cMLM)was used for genome-wide association analysis(GWAS)of monoecious traits in spinach using Genome Association and Prediction Integrated Tool(GAPIT)software. And a strong correlation region was detected on the chromosome 4. Based on the size of linkage disequilibrium block(31.2 kb),Xm gene was located in 64.6 kb region. Three genes,Spo24600,Spo24601 and Spo24602,were located in this region. In this study,KASP marker was developed in the correlation region,which can be used for the identification of monoecious spinach.

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  • Identification and Difference Analysis of the Alternative Splicing Event in the Hermaphroditic Flowers and Male Flowers of Asparagus officinalis
  • HUANG Ling*,LI Qiongying*,WEI Shugu,LAI Jia,DAI Shundong,ZHANG Qianfang,ZENG Hualan, LIU Jia,and YE Pengsheng**
  • Acta Horticulturae Sinica. 2019, 46(8): 1503-1518. DOI:10.16420/j.issn.0513-353x.2018-1025
  • Abstract ( 153 ) HTML ( 416 ) PDF (1639KB) ( 416 )    
  • Alternative splicing plays an important role in plant growth and development,which causes the same mRNA precursor for turning into different isoforms and producing protein diversity. To analyze the relationship of alternative splicing of Asparagus officinalis L. and the sex differentiation,the alternative splicing occurring in hermaphroditic flower buds and male flower buds were identified using RNA-seq technology. The results showed that a large number of alternative splicing events were identified in hermaphroditic flower buds and male flower buds. The dominated alternative splicing types were the 3′ splice site selection and exon skipping. The GO annotation showed that the shared alternative splicing genes by hermaphroditic flower buds and male flower buds mainly enriched in metabolic processes,biosynthetic process,cellular components and material-associated entries. The results of GO and KEGG analysis showed that the specific alternative splicing genes were significantly enriched in metabolic pathway,nitrogen compound metabolic process,peroxisome and oxidative phosphorylation pathway for hermaphroditic flower buds. The nitrogen compound metabolic process and the activities of antioxidant enzymes were probably related to the development of the asparagus pistil. For male flowers,the specific alternative splicing genes were significantly enriched in metabolic pathways,biosynthesis of secondary metabolites and the lipid metabolic process such as fatty acid biosynthesis. There was a possibility that the lipid metabolic process could lead to the suspension of pistil development in male flowers of asparagus. The results of this study not only provided a theoretical basis for regulating the formation of hermaphroditic flowers,but also for studying deeply the molecular mechanism of sex differentiation and sex determination in Asparagus officinalis L.

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  • The Relationship Between Enzymatic Browning and Relevant Physiological Index of Potato Tubers
  • WANG Li1,2,WANG Wanxing3,SUO Haicui2,HU Xinxi1,QIN Yuzhi1,ZENG Lu1,LI Xiaobo2,*,and XIONG Xingyao1,3,*
  • Acta Horticulturae Sinica. 2019, 46(8): 1519-1530. DOI:10.16420/j.issn.0513-353x.2018-1026
  • Abstract ( 386 ) HTML ( 585 ) PDF (4055KB) ( 585 )    
  • The characteristic of potato tubers browning seriously affect the quality of processed products and the development of processing industry. In this study,the browning-degree(BD),polyphenol oxidase(PPO),peroxidase(POD),total phenol content of fifty potato varieties were measured to investigate the relationship between enzymatic browning of tubers and PPO,POD,total phenol content by using the methods of correlation analysis,cluster analysis,and membership function. The results showed that the BD of potato tubers was significantly correlated with PPO and POD activity,but not with total phenol content. Furthermore,fifty potato varieties(lines)were divided into four types:Ⅰ(Highly browning-susceptible,HS)included one variety;Ⅱ(Browning-susceptible,BS),included nineteen varieties;Ⅲ(Middle browning-susceptible,MS),included fifteen varieties;Ⅳ(Browning-resistance,R),included fifteen varieties. Most varieties are browning-susceptible. Of all,‘Xingjia 2’and‘Jizhang 8’belonging to highly browning-resistance type,which could be used as browning-resistant resource for genetic breeding.‘Dianshu 6’belonged to highly browning-susceptible type. It could be used as a good material for the study of enzymatic browning mechanism.

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  • Identification and Expression Analysis of the syntaxin Family Genes Response to Powdery Mildew Infection in Melon
  • DONG Yumei,SUN Jianlei,WANG Chongqi,XIAO Shouhua,GAO Chao*,and JIAO Zigao*
  • Acta Horticulturae Sinica. 2019, 46(8): 1531-1542. DOI:10.16420/j.issn.0513-353x.2018-0963
  • Abstract ( 162 ) HTML ( 495 ) PDF (2040KB) ( 495 )    
  • The aim of this study was to gain the information about syntaxin family genes involved in resisting to powdery mildew infection in melon. A total of 17 syntaxin family genes were identified in the genome of melon using bioinformatics approach,which were randomly and unevenly distributed on nine respective chromosomes. Phylogenetic analysis indicated that melon syntaxin proteins could be classified into seven main groups(SYP-1,SYP-2,SYP-3,SYP-4,SYP-5,SYP-7,SYP-8),suggesting that each syntaxin functions in different pathways. This gene family in melon contained 1–12 introns. Besides,10 syntaxin genes significantly expressed in almost all tissues and seven syntaxin genes expressed only in specific tissue. Furthermore, nine syntaxin genes were up or down-regulated following powdery mildew infection and nine genes were co-expressed with CmMLO family genes in melon,indicating these genes might play important roles in resisting to powdery mildew disease in melon.

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  • Characterization and Expression Analysis of Petunia PhZPT2-12 Transcription Factor Related to Cold Response
  • ZHANG Huilin,ZHU Wan,TIAN Li,and ZHANG Wei*
  • Acta Horticulturae Sinica. 2019, 46(8): 1543-1552. DOI:10.16420/j.issn.0513-353x.2018-0891
  • Abstract ( 178 ) HTML ( 485 ) PDF (2187KB) ( 485 )    
  • Gene expression microarray of petunia was applied to select cold responsive genes,from which a C2H2-type zinc-finger protein gene PhZPT2-12 was discovered. Using inbred line‘H’as the template,PhZPT2-12 was isolated. It contains an Open Reading Frame(ORF)of 525 bp,encoding a peptide of 174 aa with two classical C2H2 domains. Phylogenetic tree analysis demonstrated that PhZPT2-12 is closely related to SlZF3 reported. Subcellular localization and transactivation activity assays showed that PhZPT2-12 was targeted to nucleus and owned no transcriptional activation activity. Semi-quantitative RT-PCR analysis revealed that the expression of PhZPT2-12 was higher in roots,stems and leaves,whereas much lower in mature flowers under normal growth conditions. Stress induced expression analysis showed that the expression of PhZPT2-12 could be induced by cold rapidly,also by salt and drought in different degrees. It is suggested that PhZPT2-12 is associated with the responses to cold,salt and other abiotic stresses.

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  • Identification and Genetic Diversity Analysis of Interspecific F1 Hybrids Between Chrysanthemum nankingense × C. lavandulifolium
  • LIU Yingxin,LI Peitong,CHI Tianhua,WANG Haibin,GUAN Zhiyong,FANG Weimin,CHEN Fadi,and ZHANG Fei*
  • Acta Horticulturae Sinica. 2019, 46(8): 1553-1564. DOI:10.16420/j.issn.0513-353x.2018-1056
  • Abstract ( 263 ) HTML ( 517 ) PDF (2973KB) ( 517 )    
  • SSR and SRAP markers and morphological traits were used to analyse the genetic diversity of the interspecific F1 population derived from the cross between diploid Chrysanthemum related species C. nankingense and C. lavandulifolium. The results showed that 122 of 131 F1 hybrids were true hybrids,and the true hybrids rate was 93.13%. Forty-two SSR primer combinations amplified 123 polymorphic bands in C. nankingense,C. lavandulifolium and 122 F1 hybrids,with an average of 3 polymorphic bands per primer combination. Eighteen SRAP primer combinations amplified 55 polymorphic bands,with an average of 3 polymorphic bands per primer combination. The genetic similarity coefficients between the interspecific hybrids of C. nankingense × C. lavandulifolium were between 0.44 and 0.90,and the phenotypic variation coefficients of interspecific hybrids were between 10.16% and 16.67%. Some transgressive individuals were observed,indicating an abundant genetic variation in the interspecific F1 population. In addition,it was observed that the interspecific hybrids segregated towards the female parent in leaf morphology. UPGMA cluster analysis based on phenotypic traits and molecular markers both classified parents and 122 hybrid progenies into 7 groups. Most of the hybrid progenies were clustered together with the female parent(C. nankingense),while the male parent(C. lavandulifolium)and a few hybrid lines were clustered separately. The clustering results showed that the F1 hybrids were more similar to the female parent. The average coefficient of genetic similarity(0.62)between female and interspecific hybrids was higher than that of male parent(0.54). This indicates that the interspecific hybrids are closer to female parent in heredity,consistent with the results of morphological analysis.

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  • Studies on the Flower Bud Differentiation of Clematis glauca
  • YANG Yi,WANG Kuiling,LIU Qinghua,JIANG Xinqiang,HAO Qing,and LIU Qingchao*
  • Acta Horticulturae Sinica. 2019, 46(8): 1565-1575. DOI:10.16420/j.issn.0513-353x.2018-0971
  • Abstract ( 271 ) HTML ( 526 ) PDF (4192KB) ( 526 )    
  • A natomic observation was made in the flower buds of Clematis glauca during flower bud differentiation with the paraffin-cut section. The results showed that flower bud differentiation of C. glauca could be divided into the undifferentiation phase,the initial differentiation phase,the primordial differentiation phase,the stamen differentiation phase,and the pistil differentiation phase,during the morphological differentiation period. The primordia of all floral organs initiated and developed centripetally. During the period of sexual cell differentiation and maturation,the male gametophytes matured faster than female gametophytes. The development of anther wall was the monocotyledonous type. Tetrasporangiate anther and glandular tapetum could be observed in C. glauca. Microsporocyte cytokinesis was successive,and microspores tetrads were mostly tetrahedral and occasionally symmetrical. In mature anther,the epidermis was degenerate completely or with a thin layer of remains left,and the endothecium was fibrous thickenings. Pollen grains of C. glauca were 2-celled. There was one chamber with few degraded ovules and a single normal one which was anatropous,unitegmic,tenuinucellate,and Polygonum type of embryo-sac with a linear tetrad of megaspores and dikaryocyte antipodal cells. The species might belong to evolutionary and transitional population relatively in the genus Clematis.

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Research Notes

  • Observation on Flower Bud Differentiation and Flowering Process of ‘Hass’Avocado(Persea americana)
  • LI Juan1,*,SI Yuanyuan1,*,CHEN Weiming2,LUO Xiaoyan1,CHEN Jiezhong2,**,and ZHANG Dengjie1
  • Acta Horticulturae Sinica. 2019, 46(8): 1585-1592. DOI:10.16420/j.issn.0513-353x.2019-0342
  • Abstract ( 294 ) HTML ( 607 ) PDF (4765KB) ( 607 )    
  • The flower bud differentiation and the flowering process of‘Hass’avocado were studied by means of the paraffin section technique and the direct observation method. The results showed that,in Guangzhou,the flower bud differentiation of the terminal shoot of‘Hass’avocado began in mid-late December,which was divided into four stages:inflorescence differentiation,perianth differentiation,stamen differentiation and pistil differentiation. Individual inflorescences differentiated from top to bottom,and apical flowers were the first to complete differentiation. The single flower of‘Haas’avocado has the characteristic of twice flowering,which opens and closes twice within 36 hours,and there is the isomorphism of pistil and stamen. The first flowering time was 6:30–10:30 and 10:30–12:30 was closed. The pistil matured at this stage and had the ability of pollination. The second flowering time was 9:30–14:30 at the next day,14:30–16:40 was closed. At this stage,the filaments elongated,stamens matured and pollinated outward. There is a partial overlap between the two flowering times,and pollination tree varieties can be configured on the basis of the two times of flowering.
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  • Investigation and Comprehensive Evaluation on Cluster Shape of Grape Germplasm Resources
  • LU Yanan1,*,ZHUGE Yaxian1,*,PEI Qingyuan1,MA Wudan1,FAN Xiucai2,LIU Chonghuai2,GUAN Le1,*,and FANG Jinggui1
  • Acta Horticulturae Sinica. 2019, 46(8): 1593-1603. DOI:10.16420/j.issn.0513-353x.2018-1071
  • Abstract ( 173 ) HTML ( 634 ) PDF (1863KB) ( 634 )    
  • The present study investigated the cluster shape of 234 varieties of grape germplasm resources. According to the“Descriptors and Data Standard for Grape(Vitis L.)”,we further categorized the grape cluster shape into the following five classes:long cone,short cone,long cylinder,short cylinder and branch. Among them,varieties with the long-cone clusters are dominant(34.61%),followed by the short-cone(33.33%),and the branched varieties are the least(5.13%). The cluster shape of table grape is mainly long conical,while the shape of wine grape is mainly short conical. The probability grading method was adopted to determine the hierarchical criterion of cluster length,width,weight and size. Using the above four traits as parameters,the fuzzy synthetic evaluation method evaluated the cluster appearance of the grape germplasm resources by giving each cultivar a specific score. The results of weighted evaluation and equal weight evaluation were generally consistent,and some varieties with good appearance,such as ‘Tensyū’,‘Cardinal’and‘Triumph’,were obtained. This study not only enriches the grape germplasm resource evaluation,but also provides a theoretical basis for further utilization of grape germplasm resources to breed new varieties.

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  • Analysis of AP3 Homologues In Cucurbitaceous Plant Unisexual Flower Development
  • CAI Guanghua1,LU Hongfeng2,HUANG Sanwen3,*,and SUN Jinjing2,*
  • Acta Horticulturae Sinica. 2019, 46(8): 1604-1615. DOI:10.16420/j.issn.0513-353x.2019-0223
  • Abstract ( 209 ) HTML ( 596 ) PDF (3919KB) ( 596 )    
  • Previous study demonstrates that CsAP3,the B class floral organ identity gene in cucumber,participate in the process of stamen arrest in female flower by regulating the expression of ethylene receptor CsETR1. To further understand the conserved role of ethylene in stamen arrestment,AP3 homologue genes in Momordica charantia,Luffa cylindrica,Cucumis melo,Citrullus lanatus,Cucurbita maxima and Cucurbita pepo were analyzed in this study. The sequences of AP3 homologous genes in Momordica charantia and Luffa cylindrica were obtained by degenerate PCR,and the homologous sequences in Cucumis melo,Citrullus lanatus,Cucurbita maxima and Cucurbita pepo were obtained from the published genome data. Sequence alignment revealed that there are non-synonymous mutations in the coding region of the short GV repeats in the C-terminus of AP3 protein in these species. Electrophoretic mobility shift assay(EMSA)and dual luciferase transient expression assay in tobacco leaves showed that,like the cucumber CsAP3 protein,the AP3 homologous proteins of melon,watermelon and pumpkin have potential to bind the ETR1 promoter,and positively regulate the ETR1 homologous gene in the corresponding species. This study provides a new angle for the study of the development of unisexual flower,and supplies important information for revealing the origin of the unisexual flower of Cucurbitaceous plant.

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New Technologies and New Methods

  • Establishment and Application of Multiplex PCR Rapid Detection of Four Citrus Viruses
  • HUANG Aijun*,WANG Ying,DING Min,LU Zhanjun,and YI Long
  • Acta Horticulturae Sinica. 2019, 46(8): 1616-1622. DOI:10.16420/j.issn.0513-353x.2018-0782
  • Abstract ( 231 ) HTML ( 466 ) PDF (1363KB) ( 466 )    
  • Citrus tristeza virus(CTV),Citrus tatter leaf virus(CVLT),Citrus yellow vein clearing virus(CYVCV),and Citrus leaf blotch virus(CLBV)are important graft-transmissible pathogens of citrus. Rapid and accurate detection methods are of great significance for the prevention and control of viral diseases. Four compatible sets of primers specific for each virus were designed based on conserved sequences of coat protein gene for multiplex PCR assay. The crucial factors of multiple PCR including primer concentration and annealing temperature were optimized for the highest sensitivity and specificity. Four specific fragments were simultaneously amplified in one PCR reaction. Their molecular weights were determined to be 889(CTLV),612(CYVCV),462(CTV)and 294 bp(CLBV). The sensibility assay showed that the sensibility of this one-step multiplex PCR is about 10 times lower than that of regular single RT-PCR. Finally,this detection system was used to detect these viruses from 59 field samples. The results revealed that the detection rate of these viruses was between 11.9% and 54.2%. In conclusion,this one-step multiplex PCR system is suitable for rapid detection of the 4 citrus viruses from field samples with accuracy,rapidity and sensitivity.

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New Cultivars

  • ‘Wenye 6’of a New Coconut Cultivar
  • SUN Chengxu*,FAN Haikuo,ZHANG Jun,and LIU Rui
  • Acta Horticulturae Sinica. 2019, 46(8): 1623-1624. DOI:10.16420/j.issn.0513-353x.2018-0437
  • Abstract ( 390 ) HTML ( 379 ) PDF (3284KB) ( 379 )    
  • ‘Wenye 6’of a new coconut cultivar was bred from Vietnam’s dwarf variety through directive breeding. This cultivar is a dwarf type,with average plant height 12–15 m,single fruit weight about 800–1 200 g,green pericarp,round fruit,thin coconut shell and pulp,kernel fruit weight about 425–550 g,coconut juice with high sugar and protein content. Thus it is suitable as fresh fruit with high edible rate. It comes into blossom and bear fruits 3–4 years after planting. The average yield is about 130 fruits per plant and the high yield could be 350. The high yield stage is in 6 years after planting.

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  • A New Cauliflower Cultivar‘Yunsonghua 1’
  • YANG Long,LI Yirong,SU Yinling,WANG Lingmin,CHEN Guangping,MA Jixian,TANG Zhengfu,DAN Zhong,and MU Wanfu*
  • Acta Horticulturae Sinica. 2019, 46(8): 1625-1626. DOI:10.16420/j.issn.0513-353x.2018-0450
  • Abstract ( 225 ) HTML ( 279 ) PDF (904KB) ( 279 )    
  • ‘Yunsonghua 1’is a new F1 hybrid of loose-curd cauliflower crossbred by a self- incompatible line‘026-2-1’as female parent and another self-incompatible line‘001-1-1’as male parent. The plant grows normally and has strong heat-resistance. It takes 60 d from transplant to harvest in spring. The leaf shap is long ellipse,the curd is loose with light-green stalk,and the average head weight is 1 000 g. This cultivar is suitable to be grown in both spring and autumn in the southern area of the Yellow River,and planting in summer in high altitude zone of Yunnan Province.

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  • A New Idesia polycarpa Cultivar‘Exuan 1’
  • CHEN Yaobin1,2,SHI Kaiming1,2,ZHENG Xiaojiang1,2,*,CAO Jian3,DONG Jingzhou1,2,and LIU Hanzhen2
  • Acta Horticulturae Sinica. 2019, 46(8): 1629-1630. DOI:10.16420/j.issn.0513-353x.2018-0456
  • Abstract ( 164 ) HTML ( 448 ) PDF (1360KB) ( 448 )    
  • ‘Exuan 1’is an Idesia polycarpa cultivar of superior quality selected from natural resource investigation in Lichuan,Hubei Province. It has fast growth,good tree shape,early fruit bearing in clusters on the branches. Its ornamental period is from October to February next year,wigh high and stable yield,strong adaptability. The single panicle weight was 207.5 g,and its oil content can reach up to 33.4%,of which the linoleic acid content is up to 63.9%. The fruit yield of 7-year-old tree is 22.5 kg per plant.

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