https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (2): 310-320.doi: 10.16420/j.issn.0513-353x.2019-0721

• Research Papers • Previous Articles     Next Articles

Molecular Cloning and Functional Characterization of Linalool Synthase Gene OfTPS5 in Osmanthus fragrans‘Lianzi Dangui’Flowers

LIU Cai1,4,*,XI Wan1,2,*,YUAN Jinmei1,2,ZHU Linlin1,2,CHEN Hongguo3,ZOU Jingjing3,ZHENG Riru1,2,**,and WANG Caiyun1,2   

  1. 1Key Laboratory for Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China;2Key Laboratory of Urban Agriculture in Central China,Ministry of Agriculture and Rural Affairs,P. R. China,Wuhan 430070,China;3Hubei University of Science and Technology,Xianning,Hubei437100,China;4Sichuan Science and Technology Extensive Station of Forestry and Grassland,Chengdu 610000,China
  • Online:2020-02-25 Published:2020-02-25

Abstract: This study was conducted to isolate a linalool synthase gene from Osmanthus fragrans Lour.‘Lianzi Dangui’based on genome sequences. Functional identification,expression analysis and the relation with aroma compound formation were investigated. The gene was designated as OfTPS5(GenBank accession No. MN366471). Sequence analysis demonstrated that the open reading frame(ORF)was 1 770 bp,and putatively encoded 589 amino acids. The OfTPS5 contained conserved domains and belonged to TPS-g subfamily. Multiple sequence alignment analysis demonstrated that it has approximate 50% homology with other plant TPSs. The transcript expression of OfTPS5 reached high level at the full bloom stage with a significant circadian rhythm,which also showed positive correlation with the aroma compound formation. Further transient expression in tobacco leaves proved that OfTPS5 was capable of producing linalool. Thus,OfTPS5 might play an important role in terpenoid biosynthesis in O. fragrans flowers.

Key words: Osmanthus fragrans, aroma, linalool and its derivatives, linalool synthase gene, expression analysis, functional characterization

CLC Number: