Abstract: The experimental base was arranged in 25-year-old orchards of Dashaling，Fengmaozhai and Wantou Villages of Yantai City，Shandong Province. Allium fistulosum，Brassica juncea and Triticum aestivum was rotated in a year. Then the continuous planting soil and the soil after rotation in each region were transported back to pot experiment. The effects of different soil treatments on seedling growth and soil environment of Malus hupehensis Rehd.（apple rootstock）were studied under pot experiment conditions，and the alternately continuous planting soil treated with methyl bromide fumigation was used as a high standard control. The results showed that after alternately continuous planting of three kinds of plants，the biomass of Malus hupehensis Rehd. seedlings increased significantly，and the fresh and dry weight of the lower part crop was increased by 1–2 times；the respiration rate of the root system was increased by more than 1 time and the bacteria was increased by 148.47%，198.57% and 165.44% in three apple orchards respectively. The fungi was reduced by 45%，which increased the bacterial/fungus ratio by a factor of 4；in addition，the soil of the seedlings enzyme activity increased；phenolic acids decreased to varying degrees，with the greatest reduction in phlorizin，which decreased by 86.67%，46.12%，and 86.31%，respectively. In addition，the copy number of F. oxysporum was also reduced by 76.34%，49.22%，and 70.89% respectively.

Key words: apple, Malus hupehensis, rotation, soil quality, biomass, microbes, phenolic acid