Cloning of MdNAC9 and Functional of Its Regulation on Flavonol Synthesis

doi:10.16420/j.issn.0513-353x.2018-1070

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (11): 2073-2081.doi: 10.16420/j.issn.0513-353x.2018-1070

• Research Papers • Next Articles

Cloning of MdNAC9 and Functional of Its Regulation on Flavonol Synthesis

SUN Qingguo，JIANG Shenghui，FANG Hongcheng，ZHANG Tianliang，WANG Nan，and CHEN Xuesen*

College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Tai’an，Shandong 271018，China

Online:2019-11-25 Published:2019-11-25

Abstract

Abstract: In order to explore the mechanism of flavonol synthesis in red flesh apple，we analyzed the pathway-related genes using mature red flesh apples，and cloned a transcription factor gene MdNAC9. The correlation between MdNAC9 and flavonol accumulation was explored by Real-Time PCR，yeast one-hybrid and luciferase reporter assay. The results showed that the expression of MdNAC9 was positively correlated with MdFLS，a key gene in flavonol synthesis，in fruit and over-expressed callus （OENAC9）. Yeast one-hybrid showed that MdNAC9 could specifically bind to MdFLS promoter. Luciferase reporter assay proved that MdNAC9 could promote the expression of MdFLS. MdNAC9 may promote flavonol accumulation by activating MdFLS.

Key words: apple, NAC transcription factor, yeast one-hybrid, luciferase reporter assay, subcellular localization, flavonol

CLC Number:

S 661.1

SUN Qingguo，JIANG Shenghui，FANG Hongcheng，ZHANG Tianliang，WANG Nan，and CHEN Xuesen*. Cloning of MdNAC9 and Functional of Its Regulation on Flavonol Synthesis[J]. ACTA HORTICULTURAE SINICA, 2019, 46(11): 2073-2081.

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Cloning of MdNAC9 and Functional of Its Regulation on Flavonol Synthesis

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