The Strcture，Genome-Wide Distribution and Expression Analysis of Pectin Methylesterase Inhibitor Genes in Brassica oleracea

doi:10.16420/j.issn.0513-353x.2019-0028

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (10): 1973-1988.doi: 10.16420/j.issn.0513-353x.2019-0028

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The Strcture，Genome-Wide Distribution and Expression Analysis of Pectin Methylesterase Inhibitor Genes in Brassica oleracea

BAI Xiaojing1，WANG Yukui1，ZUO Tonghong1，LIU Qianying1，LIAN Xiaoping2，ZHANG Hecui1，ZHANG Yizhong1，PU Min1，LUO Shaolan1，and ZHU Liquan1,*

1College of Agronomy and Biotechnology，Southwest University，Chongqing 400700，China；2College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400700，China

Online:2019-10-25 Published:2019-10-25

Abstract

Abstract: In order to clarify the number of members，structures and distribution of BoPMEI family，and find out the relationship between BoPMEI family and self-incompatibility，transcriptome data and bioinformatics approaches were used to analyze the gene structure and identify the whole genome. The expression profile of BoPMEI family genes was analyzed，and it was verified by quantitative real-time PCR. The results showed that a total of 101 BoPMEI genes were identified and they were divided into 11 groups. The expansion of BoPMEI family genes distributed unevenly on 9 chromosomes were mainly caused by whole genome triplication and tandem duplication. The divergence time was determined to be 15.56 million years ago. Among the 101 genes，9 genes，such as BoPMEI56，BoPMEI54，BoPMEI32，BoPMEI29，BoPMEI78，BoPMEI30，BoPMEI57，BoPMEI58 and BoPMEI84，had significant expression differences. qRT-PCR analysis revealed that the mRNA expression patterns of BoPMEI was similar to the RNA-seq analysis. These results indicated that at least 9 of 101 gene members were involved in the regulation of self-pollination and cross-pollination of Brassica oleracea in different ways and may be related to the self-incompatibility of Brassica oleracea.

Key words: Brassica oleracea, BoPMEI family genes, bioinformatics, expression analysis

CLC Number:

S 635

BAI Xiaojing1，WANG Yukui1，ZUO Tonghong1，LIU Qianying1，LIAN Xiaoping2，ZHANG Hecui1，ZHANG Yizhong1，PU Min1，LUO Shaolan1，and ZHU Liquan1,*. The Strcture，Genome-Wide Distribution and Expression Analysis of Pectin Methylesterase Inhibitor Genes in Brassica oleracea[J]. ACTA HORTICULTURAE SINICA, 2019, 46(10): 1973-1988.

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The Strcture，Genome-Wide Distribution and Expression Analysis of Pectin Methylesterase Inhibitor Genes in Brassica oleracea

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