Abstract: Glucosinolates are important secondary metabolites in cruciferous plants，and their derivatives and degradation productions play a special function in plant defense reaction，formation of special flavor and cancer resistance. The glucosinolate biosynthesis is complex，including side chain elongation of amino acids，formation of the core glucosinolate structure and secondary modi?cations of the amino acid side chain，involving BCATs，MAMs，CYP79s，CYP83s，AOPs and other gene families. With gradual revelation of metabolic mechanism of glucosinolate，the transcriptional regulation model has become a hot topic in metabolic mechanism research. In this paper，three important transcription factors involved in glucosinolate metabolism were summarized，among which MYB transcription factors（MYB28，MYB29，MYB76，MYB34，MYB51，and MYB122）played major regulatory roles in glucosinolates biosynthesis. And MYB28 and MYB34 are the major genes on regulating aliphatic and indole glucosinolates biosynthesis，respectively. bHLH transcription factors （MYC2，MYC3 and MYC4） have regulating function on glucosinolate biosynthesis by conjunction with MYB transcription factors. WRKY transcription factors（WRKY18 and WRKY40）synergize with CYP81F2 to negatively regulate the biosynthesis of indole glucosinolates. In addition，the responsion of transcription factors to exogenous abiotic or biotic stimulation，and the regulation mechanism on glucosinolate biosynthesis are briefly introduced in this paper. The research on transcription factors can further enrich regulatory mechanism of glucosinolate biosynthesis and provide new idea and method for molecular breeding，high-quality cultivation，biological control of cruciferous vegetable crops with high glucosinolate content.

Key words: Cruciferae, glucosinolate, transcription factor, secondary metabolism, transcriptional regulation