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ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (6): 1172-1182.doi: 10.16420/j.issn.0513-353x.2018-0903

• Research Notes • Previous Articles     Next Articles

Chromosome Karyotype Analysis and Development of SSR Molecular Markers in Toona sinensis

YU Pengfei1,2,SUN Xiaojian2,LI Chenchen2,ZHANG Xu2,ZHENG Wei2,and LIU Changjin1,2,*   

  1. 1Institute of New Rural Development,Tianjin University of Science & Technology,Tianjin 300457,China;2College of Food Engineering and Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China
  • Online:2019-06-25 Published:2019-06-25

Abstract: Chromosome karyotype analysis and SSR molecular marker development of Toona sinensis were conducted. The karyotype analysis result showed that the number of chromosomes in both populations was 2n = 56,and all of karyotypes for the populations was 2A type,the chromosome of T. sinensis was relatively primitive with little variation. 66 921 Unigenes were obtained by transcriptome sequencing analysis from T. sinensis. A total of 13 324 SSRs were identified from 11 184 Unigenes. The frequency of SSRs from these Unigenes was 19.91%,and the mean distribution distance of loci was 3.84 kb. Meanwhile,the major types such as mononucleotide,dinucleotide and trinucleotide accounted for 62.33%,19.66% and 15.99%,respectively. Furthermore,A/T was the predominant mononucleotide repeat type(62.23%),AG/CT was the predominant dinucleotide repeat type(10.31%),and AAG/CTT was the predominant trinucleotide repeat type(4.85%). 8 218 pairs of SSR primers were found by Primer 3.0. Randomly 19 pairs of primers were selected for PCR amplification by 17 T. sinensis samples,and 6 PCR products showed clear and reproducible results indicating the polymorphism among these samples.

Key words: Toona sinensis, chromosome, karyotype analysis, SSR, transcriptome

CLC Number: