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ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (1): 121-134.doi: 10.16420/j.issn.0513-353x.2018-0226

• Research Notes • Previous Articles     Next Articles

Transcriptome Studies the Effect of Glucose on Carbon Metabolism of Grape Plantlets

LIANG Guoping,LI Wenfang,MA Zonghuan,ZUO Cunwu,CHU Mingyu,MA Lijuan,HE Honghong,WAN Peng,CHEN Baihong,and MAO Juan*   

  1. (College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)
  • Online:2019-01-25 Published:2019-01-25

Abstract: The effects of glucose metabolism and photosynthesis on‘Red Globe’grape plantlets were studied under different concentrations(0,1%,2% and 4%)of exogenous glucose by the transcriptome sequencing. The result showed that the growth status of grape plantlets was the best under 2% glucose treatment,the second was 4% and 1%,the control(0)was the weakest in all treatments. 21.53 Gb Clean Data was obtained by sequencing in 4 different concentrations of glucose,and the base ratio of Q30 was more than 89.49%. Compared with reference genome,the sequence alignment efficiency was between 68.31% and 73.71% in all samples. The transfer efficiency of Single Nucleotide Polymorphisms(SNPs)ranged from 64.73% to 66.76%,and transversion was in 33.24%–35.27%. The most frequent types of mutations were A?G and C?T. The main event that occured in alternative splicing type was that the first and last exon were spliced. By setting a threshold to screening differentially express genes(DEGs),a total of 3 272 DEGs were obtained,including 2 826 down-regulated genes and 446 up-regulated genes. In the COG annotation classification,1%,2% and 4% were compared to the control,and annoted to 1 449,846 and 597 DEGs respectively,most of them are transcriptionally related genes except for general functional prediction. By detecting enzymes related leaf-carbon metabolism,SS and G6PDH activities were decreased first,then increased when the glucose concentration was added more. SS and G6PDH activities reached their peak value at 4% and 0,respectively. NI and SPS had same trend and both of their activity peak approached under the concentration of 2%. There was a consensus between expressional trend and sequenceing of 12 genes among 14 genes,indicating different concentrations of glucose regulate the expression of genes related leaf-carbon metabolism to improve the growth of‘Red Globe’.

Key words: grape, transcriptome, glucose, plantlet

CLC Number: