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ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (12): 2277-2290.doi: 10.16420/j.issn.0513-353x.2018-0158

• Research Papers •     Next Articles

Identification of S-genotypes of 20 Apple Cultivars

DING Tiyu1,*,WU Mengmeng1,*,ZHANG Ruiping1,**,YAN Zhenli1,**,YAN Yaru2,CHEN Dixin2,ZHANG Hengtao1,and GAO Qiming1   

  1. 1Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;2Henan University of Science and Technology,Luoyang,Henan 471003,China
  • Online:2018-12-25 Published:2018-12-25

Abstract: To identify 20 apple germplasms’ S-genotypes by PCR and sequencing,the universal primers and 19 pairs of specific primers of apple S-alleles were designed for‘Fuji’,‘Huarui’,‘Huashuo’,‘Huaxing’and so on,based on the highly conserved amino acid sequences FTQQYQ and anti-1/MIWPNV and the polymorphic sequences of S-alleles. To verify the accuracy of identified S-genotypes,reciprocal crosses-pollination experiments were carried out using‘Huarui’and ‘Huashuo’with‘Meiba’,‘Jinxiuhong’and‘Huaguan’,respectively. PCR results showed that when using the universal primers,two specific S-allele bands were amplified only from‘Fuji’,‘Huarui’,‘Huashuo’,‘Huaxing’,‘Meiba’and‘Hongcuibao’,whose S-genotypes were S1S9,S9S24,S5S9 and S5S24. When 19 pairs of specific primers were used for amplification,two specific bands were obtained from 14 cultivars,whose S-genotypes were S10S19,S2S3,S2S5,S3S10,S2S9,S5S24,S9S10,S3S10 and S5S9. Therefore,the S-genotypes for 20 apple cultivars were:S1S9 for‘Fuji’,S9S24 for‘Huarui’and ‘Huashuo’S5S9 for‘Huaxing’,‘Meiba’and‘Hongzhenzhu’,S5S24 for‘Hongcuibao’,‘Huayu’and‘99-1-29’,S10S19 for ‘Huashuai’,S2S3 for‘Jinyu’,S2S5 for‘Zaohong’,‘Huamei’and‘Gala’,S3S10 for‘Seokwang’,S2S9 for ‘Jinxiuhong’,‘Miyu’and‘Huaguan’,S9S10 for ‘Lüjia’,S3S10 for‘Shinano Red’,respectively. The pollination experiment showed that the fruiting rate of reciprocal crosses-pollination between‘Huarui’and ‘Huashuo’ germplasm was lower than 15.52% in 2015 and 2016. The fruit ratio of reciprocal crosses-pollination between‘Huarui’and‘Huashuo’with‘Meiba’,‘Jinxiuhong’,‘Huaguan’,‘Fuji’were higher than 46.30% in 2015 and 2016. Therefore,in this experiment,the same combination of S-genotypes had a low fruit setting percentage but a higher fruit setting percentage with different S-genotypes,indicating that the results of pollination supported the results of S-genotype identification.

Key words: Malus × domestica, self-incompatibility, S-genotype

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