https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (10): 2030-2036.doi: 10.16420/j.issn.0513-353x.2018-0172

• Research Notes • Previous Articles     Next Articles

Complete Genome Sequence Analysis of Three Citrus psorosis virus Isolates

LI Min,ZHOU Tianyu,ZHANG Song,YANG Fangyun,ZHOU Yan,ZHOU Changyong,LI Zhongan*,and CAO Mengji*   

  1. Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China
  • Online:2018-10-25 Published:2018-10-25

Abstract:

Citrus psorosis virus(CPsV)caused a destructive viral disease of citrus,while viral genome information associated with CPsV is still limited to date. In this study,we obtained the complete genome sequences of three CPsV isolates CHN-1,CHN-2 and CHN-3 by using transcriptome sequencing (RNA-Seq),RT-PCR,cloning and Sanger sequencing approaches. Genome sequence analysis showed that the full length sequences of CHN-1,CHN-2 and CHN-3 were 11 282 nucleotides(nt),11 279 nt and 11 278 nt,respectively,and comprised of four open reading frames(ORFs). Nucleotide sequence similarity between genomic RNAs of the three CPsV isolates ranged from 93.48% to 95.98%,whereas their amino acid(aa)sequence similarities were from 98.18% to 98.86%. Comparing CP genes of the three isolates with six other reported CPsV-isolates,the nucleotide sequence similarities were from 85.83% to 95.23% while amino acid sequence similarities from 94.09% to 99.32%. Phylogenetic analysis indicated that the isolates CHN-1,CHN-2 and CHN-3 clustered together into a branch of evolutionary tree,suggesting they should have the same origin.

Key words: Citrus, Citrus psorosis virus, genome, phylogenetic analysis

CLC Number: