https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (8): 1523-1534.doi: 10.16420/j.issn.0513-353x.2018-0001

• Research Papers • Previous Articles     Next Articles

Effects of Overexpression of SlMYB102 on the Tomato Seed Germination and Growth

ZHANG Xu,CHEN Lichen,and REN Zhonghai*   

  1. College of Horticultural Science and Engineering,Shandong Agricultural University,Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region,Ministry of Agriculture,State Key Laboratory of Corp Biology,Tai’an,Shandong 271018,China
  • Online:2018-08-25 Published:2018-08-25

Abstract: A total of 121 R2R3-MYB transcription factors were identified in the tomato(Solanum lycopersicum L.)genome. But the biological function for most of these transcription factors was not clear. To explore the function of SlMYB102,its coding region of 1 089 bp was cloned from‘Micro-Tom’tomato(WT)by PCR. Conserved domain and phylogenetic analysis showed that SlMYB102 included two conserved domanins of MYB and shared the highest similarity with StMYB34. Subcellular localization analysis indicated that the protein encoded by SlMYB102 was located in the nucleus. SlMYB102 was expressed in all tissues,and relatively higher expression level was observed in stem,leaf and flower. Two transgenic tomato lines overexpressing SlMYB102 were obtained through Agrobacterium-mediated transformation. The germination rate of the seeds from the transgenic lines was faster than that from WT,while the plant height,leaf area,fresh weight of shoot and root were all decreased. However,the leaf epidermal cell size did not change in the transgenic lines compared with WT. Therefore,the over- expression of SlMYB102 may negatively regulate plant growth by reducing cell division in tomato.

Key words: tomato, SlMYB102, overexpression, development

CLC Number: