Isolation of the Citrus CsHB1 Gene Promoter and Its Preliminary Functional Analysis

doi:10.16420/j.issn.0513-353x.2018-0088

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (8): 1491-1500.doi: 10.16420/j.issn.0513-353x.2018-0088

• Research Papers • Previous Articles Next Articles

Isolation of the Citrus CsHB1 Gene Promoter and Its Preliminary Functional Analysis

ZHANG Mengyuan1,2，XIE Xingnan1，LI Ying1，and GE Xiaoxia1,*

1Center of Applied Biotechnology，Wuhan Institute of Bioengineering，Wuhan 430415，China；2College of Horticulture & Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China

Online:2018-08-25 Published:2018-08-25

Abstract

Abstract: Two promoter sequences（1 741 bp and 1 613 bp）of the somatic embryogenesis development-related gene CsHB1 were cloned from genomic DNA of‘Valencia’sweet orange. The sequence similarity of these two promoters is 90.7%. There is a 141 bp fragment difference between them，which contains two Box4 cis-acting elements. The cis-acting elements of promoter were analyzed and predicted using PlantCare databases. The results indicated that the CsHB1 promoter sequence included a variety of response elements，such as endosperm specific elements，meristem expression elements，light-responsive elements，heat stress responsiveness elements，and MYB binding site. In order to study the function of CsHB1 promoter，a promoter-reporter vector prCsHB1::GUS was constructed，and introduced into Arabidopsis by Agrobacterium-mediated method. A total of 13 homozygotes were obtained with prCsHB1-1::GUS，and a total of 10 homozygotes were obtained with prCsHB1-2::GUS. GUS staining activity was detected in different tissues. The results showed that two promoter vectors were transferred and expressed successfully in Arabidopsis，and the GUS activity was mainly detected in callus and anther，not in the pods，blade and seedlings.

Key words: Citrus, CsHB1, promoter, cis-acting element, GUS stainingstaining

CLC Number:

S 666

ZHANG Mengyuan1,2，XIE Xingnan1，LI Ying1，and GE Xiaoxia1,*. Isolation of the Citrus CsHB1 Gene Promoter and Its Preliminary Functional Analysis[J]. ACTA HORTICULTURAE SINICA, 2018, 45(8): 1491-1500.

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Isolation of the Citrus CsHB1 Gene Promoter and Its Preliminary Functional Analysis

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