Abstract: The effect of greenhouse cultivation on accumulation of monoterpenes in table grapes remains unclear. The early ripening table grape varieties‘Xiangfei’and‘Zao Meiguixiang’were grown in the greenhouse and open-field as the control. Headspace solid phase micro-extraction（HS–SPME）and gas chromatography–mass spectrometry（GC–MS）combined with Automated Mass Spectral Deconvolution and Identification System（AMDIS）were employed to analyze the free monoterpenes in grape berries. A total of 29 monoterpenes from mevalonate pathway were identified. The total content of monoterpenes of the two varieties increased during berry development and‘Xiangfei’grape had higher monoterpenes content than‘Zao Meiguixiang’grape at maturity. The monoterpenes content of berries under open-field cultivation were significantly higher than that in greenhouse. The monoterpene of‘Zao Meiguixiang’grape in greenhouse could be clearly distinguished from the control by principal component analysis（PCA），but the ripening‘Xiangfei’grape in greenhouse was close to the control. In general，grape berries under open-field cultivation produced higher content of limonene，(Z)-allo-ocimene，(E,Z)- allo-ocimene，linalool，β-myrcene，terpinolen，(Z)-β-ocimene，cis/trans rose oxide，β-citronellol and cis-pyran linalool oxide.

Key words: grape, greenhouse, open-field, monoterpene, accumulation