Abstract: In this study，27 members of the VvPP2C gene family were identified in the whole genome of grapes（Vitis vinifera L.），with reference to Arabidopsis thaliana PP2C gene registration sequence. The VvPP2C genes expression was analyzed by bioinformatics，microarray expression and stress analysis. The results showed that the 27 members of VvPP2C gene family could be divided into 10 subpopulations of A，C–I，K，L. The analysis of physicochemical properties showed that the number of amino acids in the VvPP2C gene family was between 181–1 084，and the theoretical pI distribution was between 4.46–8.98. The VvPP2C gene family was mainly expressed in the cytoplasm，chloroplast and nucleus through the subcellular localization analysis. The VvPP2C gene family was mainly composed of α-helix and irregular coil in secondary structure analysis. The microarray analysis showed that VvPP2C gene members of the family responded to different stress in different tissues of the grape. The inducible expression of grape plantlets was the highest in 100，50 mmol ? L-1ABA，10% NaCl and 10% PEG，at 11，8，14 and 13 times in qRT-PCR analysis，respectively.

Key words: grape, PP2Cs gene family, gene clone, gene chip expression profiling, real-time PCR