https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (6): 1089-1100.doi: 10.16420/j.issn.0513-353x.2017-0829

• Research Papers • Previous Articles     Next Articles

Exploring Variant Sites Associated with Ve-1,I-2,Mi-1 and Pto Genes in Processing Tomato by Genome-wide Association Analysis

LIU Lei,DENG Xuebin,FENG Jingjing,WANG Jing,SUN Xiaorong,SHU Jinshuai,and LI Junming*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,The Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing 100081,China
  • Online:2018-06-25 Published:2018-06-25

Abstract: To develop the high through molecular markers of Verticillium wilt,Fusarium wilt,Meloidogyne,and Bacterial speck,the core collection of 189 processing tomato accessions was analysed by genome-wide association study(GWAS). The 189 processing tomato accessions can be divided into two subgroups,by the Neighbor-joining tree and principal component analysis of the data of 0.5× re-sequence. The disease resistant genes,Ve-1,I-2,Mi-1 and Pto,were tested using molecular markers. The genome-wide association of the resistance genes were analysed by mixed linear model(MLM). The results were consistent with previous gene localization,and the inner SNPs of the resistance genes of Ve-1,I-2,Mi-1 and Pto were obtained,which can improve the accuracy of gene detection. The highest association SNP of Ve-1 was on the gene of Ve-1,and the highest loci associated with I-2,Mi-1 and Pto genes were located in the flanking region. The background and the frequency of variation of SNPs may affect the result of GWAS. The results indicated that the core collection of processing tomato can be used for exploring other traits. The SNPs obtained by GWAS can be used for the development of SNP markers,molecular markers assisted breeding and improvement of the breeding efficiency about tomato.

Key words: tomato, resistance gene, GWAS, SNP

CLC Number: