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ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (6): 1041-1053.doi: 10.16420/j.issn.0513-353x.2017-0719

• Research Papers • Previous Articles     Next Articles

Molecular Cloning and Functional Identification of Apple Auxin Repressor Protein Gene MdIAA26

HAN Pengliang,LIU Xiaojuan,LIU Xin,DONG Yuanhua,HU Dagang*,and HAO Yujin*   

  1. State Key Laboratory of Crop Biology,MOA Key Laboratory of Horticultural Crop Biology(Huanghuai Region)and Germplasm Innovation,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2018-06-25 Published:2018-06-25

Abstract: An auxin repressor protein family gene(GenBank accession number:MDP0000164095)was cloned from‘Royal Gala’apple cultivar(Malus × domestica Borkh.). It contains complete open reading frame(ORF)of 978 bp in length and encodes 325 amino acids. Phylogenetic tree analysis showed that the deduced peptides exhibited the highest sequence similarity with AtIAA26(Arabidopsis),which was also indicated as an auxin repressor. Based on the result,we named this deduced gene as MdIAA26. In silico analysis suggested that the promoter sequence of MdIAA26 contained several typical cis-acting elements,including abscisic acid-,gibberellin-,salicylic acid- and drought-responsive elements by using PlantCare Databases. qPCR assays showed that MdIAA26 constitutively expressed in different tissues of apple,with higher expression levels in roots and leaves. The expression pattern of MdIAA26 in apple tissue culture seedling was noticeably induced by exogenous ABA and IAA. Further,the MdIAA26 overexpressed apple calli were treated with exogenous ABA and ectopical expressed Arabidopsis plants were treated with exogenous ABA,PEG and IAA. The growth potential of transgenic materials were significantly stronger than the wild type(WT,the control). All this suggests that transgenic materials were more insensitive to exogenous ABA and IAA and less resistant to drought than the control.

Key words: apple, MdIAA26, AtIAA26, auxin repressor protein, abscisic acid

CLC Number: