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ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (6): 1031-1040.doi: 10.16420/j.issn.0513-353x.2017-0571

• Research Papers • Previous Articles     Next Articles

Overexpression of Apple Peptide Hormone Gene MdCEP1 Promotes the Accumulation of Anthocyanins

LI Rui,AN Jianping,YOU Chunxiang,WANG Xiaofei*,and HAO Yujin*   

  1. State Key Laboratory of Crop Biology,MOA Key Laboratory of Horticultural Crop Biology(Huanghuai Region)and Germplasm Innovation,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2018-06-25 Published:2018-06-25

Abstract: The effects of apple peptide hormone MdCEP1(C-TERMINALLY ENCODED PEPTIDE1)on the regulation of anthocyanin synthesis were investigated by using Malus × domestica‘Orin’as the test material,to provide reference for further study on the mechanism of anthocyanin synthesis and characterization of MdCEP1 function. Chromosomal localization analysis revealed that MdCEP1 was located on the chromosome 15 of the apple genome and had only one exon. Protein sequence alignment showed that CEP domains were conserved in different species. Promoter analysis showed that the MdCEP1 promoter sequence contained a number of cis-acting elements,including CAT-box elements associated with meristem,gibberellin response elements(P-boxes),light-responsive elements(MNF1)and the MYB binding site(MBSI)involved in flavonoid biosynthesis. MdCEP1 transgenic apple calli were obtained by Agrobacterium tumefaciens-mediated genetic transformation. Further analysis found that overexpression of MdCEP1 could significantly promote the anthocyanin accumulation,and promote the expression of apple anthocyanin biosynthetic genes. Ectopic expression of MdCEP1 in Arabidopsis thaliana,could also promote the accumulation of anthocyanin,and promote the expression of anthocyanin biosynthetic genes in Arabidopsis thaliana. The results showed that MdCEP1 could positively control the synthesis of anthocyanin.

Key words: apple, peptide hormone, MdCEP1, anthocyanin

CLC Number: