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ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (5): 977-987.doi: 10.16420/j.issn.0513-353x.2017-0441

• Research Notes • Previous Articles     Next Articles

Cloning and Expression Analysis of CmDREBa-2,A Resistance-related Gene to Chrysanthemum White Rust

XIONG Chaoming1,ZHAO Xinghua2,JIA Hongmei1,YAN Xin1,and MAO Hongyu1,*   

  1. (1College of Forestry,Shenyang Agricultural University,Shenyang 110866,China;2Institute of Flowers,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)
  • Online:2018-05-25 Published:2018-05-25

Abstract: Based on the information of transcriptome database derived from‘C029’,a Puccinia horiana Henn. immune Chrysanthemum morifolium cultivar,the cDNA sequence of DREB(Dehydration responsive element binding protein)was cloned. This DREB gene was named CmDREBa-2. Sequence analysis showed that the open reading frame(ORF)of CmDREBa-2 was 291 bp,encoding 96 amino acids. The molecular weight of the predicted protein was 11 159.04 and the pI value was 11.34. The protein was located in the nucleus,and it was a hydrophilic and unstable protein. The transcription factor of CmDREBa-2 contained the AP2 DNA binding domain which was composed by 49 amino acids. The results of phylogenetic analysis showed that the CmDREBa-2 belonged to the DREB family of A-1 and it had a close relationship with CmDREBa and CmDREBb from C. morifolium. The expression level of CmDREBa-2 reached a maximum at 6 h after Puccinia horiana Henn. infection,with 34 times as much as the control. We also found that the expression of CmDREBa-2 was induced by SA,MeJA and ETH.

Key words: chrysanthemum, CmDREBa-2, cloning, bioinformatics analysis, expression analysis

CLC Number: