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ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (3): 530-541.doi: 10.16420/j.issn.0513-353x.2017-0343

• Research Papers • Previous Articles     Next Articles

Proteomics Analysis of Lily Blight Disease-resistant Clones

ZHANG Yiping1,2,QU Suping2,YANG Xiumei2,MA Lulin2,XU Feng2,WANG Jihua2,*,and HE Yueqiu1,*   

  1. (1College of Plant Protection,Yunnan Agricultural University,Kunming 650205,China;2 Flower Research Institute,Yunnan Academy of Agricultural Sciences,National Engineering Research Center for Ornamental Horticulture,Yunnan Flower Breeding Key Laboratory,Yunnan Flower Engineering Center,Kunming 650205,China)
  • Online:2018-03-25 Published:2018-03-25

Abstract: To elucidate the key proteins related to disease-resistance of lily induced by Fusarium oxysporum f. sp. lilii,protein two-dimensional gel electrophoresis technology was used to analyze profiles of the disease-resistant and susceptible clones after inoculation of Fusarium oxysporum f. sp. lilii for 0,24,48 and 72 h. Tweenty-five differentially expressed proteins were successfully identified,including 10 differentially expressed proteins identified as unknown function proteins. The rest of the 15 differentially expressed proteins can be categorized into 4 classes according to the function,namely the defense reaction,photosynthesis,carbohydrate and energy metabolism,and heat shock-related proteins. The disease related proteins participated in the defense reaction including presumption of disease-resistant protein RPS2,putative late blight resistant protein homolog R1C-3,peroxidase Q,ascorbate peroxidase and NBS-LRR-like protein,which may play key roles in the resistance against lily blight.

Key words: lily, protein extraction, two-dimensional electrophoresis, Fusarium oxysporum f. sp. lilii, differential proteomics

CLC Number: