Abstract: 6-year-old‘Jingmi’grapevines grafted on‘Beta’were used to study the effect of spacing between new shoots on canopy light environment and fruit quality under protected cultivation in two consecutive years. Under the premise of controlling the same fruit load，three new shoot spacing treatments （10，15 and 20 cm）were set. The results showed that the canopy gap fraction and openness increased with the shoot spacing increase，10 cm > 15 cm > 20 cm，whilst，leaf area index（LAI）and light interception rate were opposite. Light utility efficiency of the new shoots spacing treatment 20 cm was the lowest. LAI of the treatment 10 cm was the highest which caused canopy closure. LAI of the treatment 15 cm was reasonable and light utility efficiency was higher. Single berry weight of the new shoots spacing treatments 15 and 20 cm was not significantly different，and was significantly higher than that of the treatment 10 cm. The fruit color of the new shoots spacing treatment 15 cm was the best，followed by the treatment 20 cm，and the treatment 10 cm was the worst. In terms of fruit interior quality，the contents of soluble sugar and vitamin C were the new shoots spacing treatments 15 cm > 20 cm > 10 cm. The trend of titratable acid content was opposite to the former. 10 cm treatment was the highest，and no significant difference was found between 15 and 20 cm treatments. The contents of the characteristic aroma of linalool with rose aroma and hexanal with faint and fruity scent in the new shoots treatment 15 cm were significantly higher than that of the treatments 20 and 10 cm. The contents of nerol with faint and fruity scent，geraniol with potpourri，citronellol with rose aroma and 2-hexene with faint and fruity scent in the treatments 15 and 20 cm were significantly higher than that of the treatment 10 cm. The fruit aroma of the treatment 15 cm was stronger，which can better reflect the variety characteristics. All the results showed that the new shoots spacing treatment 15 cm，horizontal dragon stem combined with V-shaped canopy of high photosynthetic-efficient and laborsaving，showed the best efficiency in the protected cultivated ‘Jingmi’grapevine.

Key words: grapevine, protected cultivation, new shoots spacing, light environment, fruit quality, GC–MS