Abstract:

Somatic embryo induction and morphology as well as histology identification were carried on Hippeastrum vittatum‘Red Lion’by the way of scales explant. The results showed that the optimum medium for embryonic callus induction was MS + 2 mg · L^-1 BA + 1 mg · L^-1 NAA + 2 mg · L^-1 TDZ + 30 g · L^-1 sucrose，and the rate of embryonic callus induction reached to 90.63%. However，there was no embryonic callus obtained in medium without TDZ. On PGR-free medium，somatic embryos developed after 15 days and plantlets with shoot and roots formed after 30 days. There were 6 types of callus：white loose callus，transparent fluffy callus，transparent compact callus，light brown crisp callus and jade green crisp callus，for embryonic callus and light brown compact callus，for non-embryonic callus. Global embryos，heart-shaped embryos and club-shaped embryos were observed in the development process of somatic embryo.

Key words: Hippeastrum vittatum, somatic embryogenesis, histological morphology identification, crabapple, traceability, evolution, classification