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ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (1): 161-167.doi: 10.16420/j.issn.0513-353x.2015-0505

• Research Notes • Previous Articles     Next Articles

Cloning and Expression Analysis of Temperature-induced Lipocalin in Narcissus tazetta var. chinensis

DING An-qi1,*,FENG Ying2,*,ZHU Li-ying1,XU Shi-rong1,QIN Jun1,and PAN Dong-ming1,**   

  1. 1Institute of Postharvest Science and Technology of Horticultural Products,Fujian Agriculture and Forestry University,Fuzhou,350002,China;2School of Resource and Environmental Science,Quanzhou Normal University,Quanzhou,Fujian 362000,China
  • Online:2016-01-25 Published:2016-01-25

Abstract:

An encoding temperature-induced lipocalin(TIL)gene NtTIL was separated and cloning by PCR in Narcissus tazetta var. chinensis. The open reading frame of NtTIL was 567 bp,encoding a protein of 188 amino acids. Biological software for NtTIL protein analysis showed that:The gene belongs to lipocalin-2 superfamily gene family;typical domains deduced from amino acid sequence contained SCR1,SCR2 and SCR3 three of botanical lipocalin. It’s homology greater than wheat,Arabidopsis and other plants 75%. Sequence analyses revealed that this protein was stable and hydrophilic without signal peptides;transmembrane movement was from outside to inside in the outward N-terminus,then transmembrane. And the relative expression of this gene was surveyed by real-time quantitative PCR during different growth stage. The results showed that its relative expression during different growth stage was different;the NtTIL expression was low in bulb swelling stage,expression increased and then reduced in dormancy stage,and expression increased slightly in flower differentiation stage. In conclusion,itsuggested that NtTIL could play a role for regulation of bud dormancy in Narcissus tazetta var. chinensis.

Key words: Narcissus tazetta var. chinensis, temperature-induced lipocalin, cloning, expression, dormancy

CLC Number: