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ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (11): 2306-2314.doi: 10.16420/j.issn.0513-353x.2015-0513

• Research Notes • Previous Articles     Next Articles

Cloning and Expression Analysis of Sulfate Transporter CsSUL3.5 Gene in#br# Tea Plant

HU Yu-rong1,YUE Chuan1,ZHOU Chao2,HUANG Yu-ting1,CAO Hong-li1,HAO Xin-yuan1,WANG#br# Xin-chao1,and ZENG Jian-ming1,*   

  1. 1Tea Research Institute of the Chinese Academy of Agricultural Sciences,National Center for Tea Improvement/Key
    Laboratory of Tea Biology and Resources Utilization,Hangzhou 310008,China;2College of Food Science,Southwest
    University,Chongqing 400715,China
  • Online:2015-11-25 Published:2015-11-25

Abstract:

The cDNA of putative sulfate transporter(CsSUL3.5)gene(GenBank accession number
KP984500)was cloned from tea plant[Camellia sinensis(L.)O. Kuntze]. Bioinformatics analysis
indicated that the cDNA of CsSUL3.5 containing 1 914 bp ORF encoded 637 amino acids residues with a
putative molecular mass of 70.38 kD. It was predicted that CsSUL3.5 was a non-secretory protein without
a signal peptide. CsSUL3.5 could be located in the plasma membrane with 11 transmembrane domains.
Further analyses showed that CsSUL3.5 had the typically conserved domain and the highest identity(74%)with Sulfate Transporter 3.5 of Nicotiana sylvestris. The real-time PCR analysis showed that
CsSUL3.5 transcripts were significantly increased upon the treatment of Na2SO4 and Na2SeO4.

Key words: tea plant, CsSUL3.5, selenium, cloning, expression analysis

CLC Number: