Abstract: RAPD and isozyme analyses by 14 primers, which are 10 oligonucleotides, and 7 isozyme systems were used to the study of the relationship among species of Paphiopedilum in China. Meanwhile the classifications of Paphiopedilum got from traditional classification (Chen's and Cribb's) , ITS (Cox's) , RAPD and isozyme (Our's) are compared in this paper. The result shows that the relationship of the subgenus revealebs isozyme dater ismore similar to traditional classification than that of RAPD dater. Our results exhibited that the Subgenus Paphioped ilum need to be subdivided into several sections. The species P. wardii, P. appletonianum, P. purpuratum, P. ‘Yunnahupi’are wallcuted its own section.

Key words: Paphiopedilum, RAPD, Isozyme, Relationship