Improvement of Photosynthetic Capacity and Lycopene Content of Tomatoes by Covering with Light Conversion Plastic Films

doi:10.16420/j.issn.0513-353x.2021-2002

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1517-1530.doi: 10.16420/j.issn.0513-353x.2021-2002

• Research Papers • Previous Articles Next Articles

Improvement of Photosynthetic Capacity and Lycopene Content of Tomatoes by Covering with Light Conversion Plastic Films

FENG Qian¹^,², DONG Lingdi³, YIN Yilei⁴, JIAO Yonggang³, GUO Jinghua³, LI Qingyun², LIU Bingwei⁵, YU Xianchang¹, SUN Mintao¹, HE Chaoxing¹, LI Yansu¹, WANG Jun¹, YAN Yan¹^,^**()

¹Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
²College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China
³Economic Crops Research Institute,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 255400,China
⁴Institute of Protected Agriculture,Academy of Agricultural Planning and Engineering,Ministry of Agriculture and Rural Affairs,Beijing 100125,China
⁵Huadun Xuehua Plastics（Gu’an）Co.,Ltd,Langfang,Hebei 065500,China

Received:2021-02-05 Revised:2021-07-26 Online:2021-08-25 Published:2021-09-06
Contact: YAN Yan E-mail:yanyan101@163.com

Abstract

Abstract:

The light conversion plastic film is formed by adding different light conversion agents into ordinary EVA（ethylene-vinyl acetate）copolymer film. In this study,we compared the effects of different plastic films on plant growth,leaf photosynthesis,yield,and fruit quality of tomato 'Jinpeng 8' in a solar greenhouse. The optical and mechanical properties（such as thickness,tensile strength,elongation at break,and right-angle tearing strength）of EVA film（control）,light conversion film C-RBI-2（EVA film plus light conversion agent VTB470）,and C-RBI-3（EVA plus light conversion agent VTB450）were measured by using a fluorometer,infrared spectrometer,and dual-beam UV-visible spectrophotometer,respectively. Our results demonstrated that C-RBI-2 film converted ultraviolet light into blue light with emission spectrum at 470 nm. Covering C-RBI-2 film increased the net photosynthetic rate（P_n）by upregulating the expression of the the small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase（RbcS）,improving the tightness of the leaf tissue structure and the leaf tissue porosity values,and increasing the thickness of fence cells,sponge cells,and leaves. Additionally,Covering C-RBI-3 film promoted the root absorption of nitrogen（N）,phosphorus（P）,and potassium（K）,increased tomato yields,and raised the lycopene content by upregulating the gene expressions of phytoene synthase 1（PSY1）,phytoene desaturase（PDS）,and ζ-carotene desaturase（ZDS）during the ripening stage. By contrast,the C-RBI-3 film converted ultraviolet light into blue light with an emission spectrum at 450 nm,inhibited the gene expressions of RbcS and RbcL,reduced the activity of P_n and Rubisco in tomato leaves,reduced the content of lycopene,and clarified the fact that the ultraviolet-to-blue light convertion with the emission spectrum at 470 nm can improve the photosynthetic capacity and lycopene content of tomato leaves. Taken together,covering C-RBI-2 is a better choice to effectively promote the photosynthetic capacity of tomato leaves and improve the fruits’ quality in the solar greenhouse when growing plants during the autumn and winter.

Key words: tomato, photosynthesis, light conversion film, blue light, solar greenhouse, quality

CLC Number:

S641.2

FENG Qian, DONG Lingdi, YIN Yilei, JIAO Yonggang, GUO Jinghua, LI Qingyun, LIU Bingwei, YU Xianchang, SUN Mintao, HE Chaoxing, LI Yansu, WANG Jun, YAN Yan. Improvement of Photosynthetic Capacity and Lycopene Content of Tomatoes by Covering with Light Conversion Plastic Films[J]. Acta Horticulturae Sinica, 2021, 48(8): 1517-1530.

Figures/Tables 12

References 40

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基因 Gene	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
RbcL	AATTGTTGGTGATGGTAGT	CCTTCTCGTAGTCTTGT
RbcS	GCAATGGTGGAAGAGTTA	AGGAAGGTATGAGAGTGT
Actin	TTCCGTTGCCCAGAGGTCCT	TCGCCCTTTGAAATCCACATC
PSY1	TGGCCCAAACGCATCATATA	CACCATCGAGCATGTCAAATG
PDS	CTGGAGGCAAGGGATGTT	TAAAGGAGCGGGTAAAGC
ZDS	GGAGTGCCTGTTGTTACCG	CTCAGACCTTGGGACGA
Actin	TGTCCCTATTTACGAGGGTTATGC	AGTTAAATCACGACCAGCAAGAT

基因 Gene	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
RbcL	AATTGTTGGTGATGGTAGT	CCTTCTCGTAGTCTTGT
RbcS	GCAATGGTGGAAGAGTTA	AGGAAGGTATGAGAGTGT
Actin	TTCCGTTGCCCAGAGGTCCT	TCGCCCTTTGAAATCCACATC
PSY1	TGGCCCAAACGCATCATATA	CACCATCGAGCATGTCAAATG
PDS	CTGGAGGCAAGGGATGTT	TAAAGGAGCGGGTAAAGC
ZDS	GGAGTGCCTGTTGTTACCG	CTCAGACCTTGGGACGA
Actin	TGTCCCTATTTACGAGGGTTATGC	AGTTAAATCACGACCAGCAAGAT

棚膜 Sample	厚度值/mm Thickness	拉伸强度/MPa Tensile strength		断裂伸长率/% Elongation at break		直角撕裂强度/（N · mm^-1） Right angle tear resistance
棚膜 Sample	厚度值/mm Thickness	纵向 Vertical	横向 Horizontal	纵向 Vertical	横向 Horizontal	纵向 Vertical	横向 Horizontal
EVA （对照 Control）	0.12 a	24.9 ± 0.46 b	26.9 ± 0.40 c	465.4 ± 8.06 c	683.9 ± 7.41 b	116.6 ± 5.42 a	109.9 ± 1.55 b
C-RBI-2	0.12 a	28.7 ± 0.40 a	30.2 ± 0.50 a	573.9 ± 6.44 a	702.8 ± 4.02 a	112.4 ± 3.40 b	113.8 ± 1.17 a
C-RBI-3	0.12 a	28.8 ± 0.36 a	29.1 ± 0.35 b	522.9 ± 3.45 b	689.6 ± 7.50 ab	110.4 ± 3.58 b	106.3 ± 1.16 c

棚膜 Sample	厚度值/mm Thickness	拉伸强度/MPa Tensile strength		断裂伸长率/% Elongation at break		直角撕裂强度/（N · mm^-1） Right angle tear resistance
棚膜 Sample	厚度值/mm Thickness	纵向 Vertical	横向 Horizontal	纵向 Vertical	横向 Horizontal	纵向 Vertical	横向 Horizontal
EVA （对照 Control）	0.12 a	24.9 ± 0.46 b	26.9 ± 0.40 c	465.4 ± 8.06 c	683.9 ± 7.41 b	116.6 ± 5.42 a	109.9 ± 1.55 b
C-RBI-2	0.12 a	28.7 ± 0.40 a	30.2 ± 0.50 a	573.9 ± 6.44 a	702.8 ± 4.02 a	112.4 ± 3.40 b	113.8 ± 1.17 a
C-RBI-3	0.12 a	28.8 ± 0.36 a	29.1 ± 0.35 b	522.9 ± 3.45 b	689.6 ± 7.50 ab	110.4 ± 3.58 b	106.3 ± 1.16 c

处理 Treatment	上表皮 Upper epidermal	下表皮 Lower epidermal	栅栏细胞 Palisade parenchyma cells	海绵细胞 Spongy parenchyma cells
EVA（对照 Control）	15.174 ± 2.536 b	8.702 ± 1.654 b	9.280 ± 1.732 ab	7.543 ± 1.544 b
C-RBI-2	19.963 ± 2.821 a	12.989 ± 2.797 a	9.710 ± 1.934 a	8.461 ± 1.354 a
C-RBI-3	14.165 ± 2.496 b	10.823 ± 2.407 b	8.772 ± 1.303 b	6.890 ± 1.092 c
处理 Treatment	叶片厚度 Thickness of leaves	CTR	SR
EVA（对照 Control）	154.346 ± 12.276 b	5.023 ± 0.427 c	3.943 ± 0.368 c
C-RBI-2	165.876 ± 13.727 a	6.002 ± 0.757 a	4.880 ± 0.671 b
C-RBI-3	173.719 ± 12.176 b	5.800 ± 0.719 b	5.073 ± 0.400 a

Improvement of Photosynthetic Capacity and Lycopene Content of Tomatoes by Covering with Light Conversion Plastic Films

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处理 Treatment	总氮含量 Total N content			总磷含量 Total P content
处理 Treatment	根 Root	茎 Stem	叶 Leaf	根 Root	茎 Stem	叶Leaf
EVA（对照 Control）	1.982 ± 0.046 b	2.099 ± 0.092 ab	3.153 ± 0.030 b	0.321 ± 0.049 b	0.648 ± 0.029 b	0.627 ± 0.026 ab
C-RBI-2	2.141 ± 0.005 a	1.919 ± 0.074 b	3.400 ± 0.062 a	0.423 ± 0.028 a	0.695 ± 0.022 a	0.697 ± 0.056 a
C-RBI-3	1.864 ± 0.025 c	2.319 ± 0.093 a	3.139 ± 0.052 b	0.297 ± 0.081 b	0.729 ± 0.022 a	0.598 ± 0.016 a
处理 Treatment	总钾含量 Total K content
处理 Treatment	根 Root	茎 Stem	叶Leaf
EVA（对照 Control）	3.103 ± 0.289 b	4.577 ± 0.323 a	2.716 ± 0.028 b
C-RBI-2	4.309 ± 0.577 a	4.429 ± 0.220 a	2.922 ± 0.090 a
C-RBI-3	2.831 ± 0.314 b	4.593 ± 0.507 a	2.411 ± 0.031 c

处理 Treatments	产量/（t • hm^-2） Yield	维生素C/（mg • kg^-1） Vitamin C	番茄红素/（μg • g^-1） Lycopene
EVA（对照 Control）	84.040 ± 1.647 ab	147.500 ± 0.200 a	2.505 ± 0.005 b
C-RBI-2	87.049 ± 1.024 a	147.400 ±0.300 a	3.347 ± 0.021 a
C-RBI-3	80.725 ± 1.522 b	138.850 ±0.350 b	2.274 ± 0.015 c

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[5]	DENG Chaojun, CHEN Xiuping, XU Qizhi, JIANG Jimou, ZHENG Shaoquan, HU Wenshun, JIANG Fan, XU Jiahui, SU Wenbing, ZHANG Yaling, and HUANG Jingfeng. A New Longan Cultivar‘Fuxiang’with Rich Aroma [J]. Acta Horticulturae Sinica, 2022, 49(S2): 77-78.
[6]	HUANG Li, CHEN Caizhi, YU Xiaolin, YAO Xiangtan, and CAO Jiashu, . A New Early-mid Maturing Chinese Cabbage Pak-choi Cultivar‘Zhedaqing’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 93-94.
[7]	ZHENG Jirong, WANG Tonglin, and HU Songshen. A New Tomato Cultivar‘Hangza 603’with High Quality [J]. Acta Horticulturae Sinica, 2022, 49(S2): 103-104.
[8]	ZHENG Jirong and WANG Tonglin. A New Tomato Cultivar‘Hangza 601’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 105-106.
[9]	ZHENG Jirong and WANG Tonglin. A New Cherry Tomato Cultivar‘Hangza 503’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 107-108.
[10]	HUANG Tingting, LIU Shuqin, ZHANG Yongzhi, LI Ping, ZHANG Zhihuan, and SONG Libo. A New Cherry Tomato Cultivar‘Yingshahong 4’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 109-110.
[11]	SUN Pingdong, WANG Hui, HU Yingxiong, KUANG Huiyun, Xu Shengyu, SHI Biao, ZHENG Hongjian, and LIN Jinyuan, . A New Sweet Corn Cultivar‘Huxuetian 1’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 149-150.
[12]	HAN Qing and SHEN Xuefang. A New Sweet and Waxy Cultivar‘Wucai Tiannuo 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 151-152.
[13]	LI Zhongjian, WANG Shaoxin, WANG Baobao, XU Luo, and FENG Jianying. A New Waxy Corn Cultivar‘Shibainuo 3’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 153-154.
[14]	WANG Shaoxin, LI Zhongjian, XU Luo, FENG Jianying, WANG Baobao, and CHEN Li. A New Waxy Corn Cultivar‘Shibainuo 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 155-156.
[15]	WEI Yuhong, LU Xiaolu, HUANG Yun, MA Yinghua, FAN Jinghua, and TIAN Jiaxi. A New Netted Muskmelon Cultivar‘Huami 303’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 165-166.