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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1695-1705.doi: 10.16420/j.issn.0513-353x.2020-0688

• Research Papers • Previous Articles     Next Articles

The Important Biological Function of T111,an Evolutional Conserved Amino Acid in FveTAA1,a Key Enzyme in Strawberry Auxin Biosynthesis

YANG Liyuan1,2, WANG Qian2, WANG Xuhui1,2, XU Tongda1,2,*(), MA Jun2,*()   

  1. 1College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350007,China
    2Research Center of Horticulture and Metabolism Biology,Taiwan Strait Joint Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350007,China
  • Received:2021-02-25 Revised:2021-05-24 Online:2021-09-25 Published:2021-09-30
  • Contact: XU Tongda,MA Jun E-mail:tdxu@sibs.ac.cn;junma@sibs.ac.cn

Abstract:

In this study,combined with bioinformatics,biochemistry and genetics methods,the biological function of the evolutionary conserved site T111 of tryptophan transaminase TAA1(Tryptophan aminotransferase of Arabidopsis 1)in strawberry(Fragaria vesca)were studied. Though gene expression analysis,it was found that strawberry FveTAA1 was up-regulated during fruit expansion and fruit ripening stages,suggesting that it may have important biological function during fruit development. Based on bioinformatics analysis,it was found that the 111th threonine(T111)of FveTAA1 protein,which is highly evolutional conserved,may have an important biological function. Through biochemical detection,it was found when T111 was mutated to alanine T111A,a simulated non-phosphorylation version,or aspartic acid T111D,a simulated phosphorylation version,FveTAA1 will lose its tryptophan aminotransferase activity,which suggests that this site maybe significant for its function. Using genetic methods,it was found that FveTAA1 WT could complement the genetic phenotype of TAA1/TAR mutant in Arabidopsis whereas FveTAA1 T111A / T111D could not,which further proved that FveTAA1 T111 site plays a key role for its biological function. Overall,these results not only support the evolutionary conservative regulation mechanism of auxin synthesis,but also provide a theoretical basis for the study of the regulation mechanism of auxin synthesis in fruit development and ripening process in strawberry.

Key words: strawberry, auxin, TAA1, transaminase activity, fruit growth and development

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