https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1680-1694.doi: 10.16420/j.issn.0513-353x.2021- 0219

• Research Papers • Previous Articles     Next Articles

Comparative Transcriptome Analysis of Different Developmental Stages of Flowers and Fruits in Loquat Under Low Temperature Stress

XU Hongxia1, ZHOU Huifen2, LI Xiaoying1, JIANG Luhua3, CHEN Junwei1,*()   

  1. 1Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
    2Zhejaing Agricultural Technology Extension Center,Hangzhou 310020,China
    3Fruit Tree Technology Extension Station of Chun’an Agricultural Bureau,Chun’an,Zhejiang 311700,China
  • Received:2021-05-24 Revised:2021-08-25 Online:2021-09-25 Published:2021-09-30
  • Contact: CHEN Junwei E-mail:chenjunwei@zaas.ac.cn

Abstract:

The study compared the low temperature response mechanism of loquat(Eriobotrya japonica Lindl.)flowers and fruits at different developmental stages. Flower buds,fully open flowers and young fruits were used as the experimental materials,treated at-3 ℃ for 12 h,and samples without low temperature treatment were used as controls. The relevant physiological and biochemical indexes,as well as transcriptome were determined both in control and treated samples. Low temperature stress led to the destruction of cell membranes,the generation rate of superoxide anion,the content of malondialdehyde and proline,and the activity of antioxidant enzymes were dramatically increased. In general,the low temperature resistance ability was flower bud > flower > young fruit. Transcriptome sequencing analysis obtained a total of 6 987 differentially expressed genes(DEG). A large number of metabolic pathways related to low temperature stress were found,including glycolysis/gluconeogenesis,glyoxylic acid and dicarboxylic acid metabolism and phosphoinositol metabolism pathways in carbohydrate metabolism,tryptophan metabolism and tyrosine metabolism pathways in amino acid metabolism,glycerol phospholipid metabolism,α-linolenic acid metabolism and glyceride metabolism pathways in ester metabolism,isoquinoline alkaloid biosynthesis pathway in secondary metabolism,as well as thiometabolism pathway in energy metabolism,were significantly enriched in all comparison groups of low temperature and control at three flower and fruit development stages,which indicated that these pathways were important metabolic pathways in response to low temperature stress in loquat. The phenylpropanoid biosynthesis pathway was significantly enriched in both flower bud and flower comparison groups,and more amino acid metabolism pathways were enriched in young fruit comparison group after low temperature treatment. In addition,53 AP2-EREBP genes,14 WRKY genes and 15 NAC genes were screened from the differentially expressed genes related to low temperature treatment,and 12 low temperature response-related transcription factor genes were analyzed by qRT-PCR,which further confirmed the accuracy of transcriptome data.

Key words: Eriobotrya japonica, flower, fruit, development stage, low temperature, transcriptome, differentially expressed gene, transcription factor

CLC Number: