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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1665-1679.doi: 10.16420/j.issn.0513-353x.2020-0636

• Research Papers • Previous Articles     Next Articles

Genome-wide Identification of the SKP1-like Family and Analysis of Their Expression During Early Somatic Embryogenesis in Longan

ZHANG Chunyu, XU Xiaoqiong, XU Xiaoping, ZHAO Pengcheng, SHEN Xu, Munir Nigarish, ZHANG Zihao, LIN Yuling, Chen Zhenguang, LAI Zhongxiong*()   

  1. Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Received:2021-02-09 Revised:2021-08-11 Online:2021-09-25 Published:2021-09-30
  • Contact: LAI Zhongxiong E-mail:Laizx01@163.com

Abstract:

Based on the amino acid sequence of Arabidopsis SKP1-like,the SKP1-like members were identified in the longan genome database to explore the molecular characteristics and its expression patterns during early somatic embryogenesis in Dimocarpus longan. In the meanwhile,bioinformatics methods were used to analyse the physical and chemical properties of its protein,system evolution characteristics,chromosome positioning,collinearity and selective pressure,gene structure,conserved motifs,protein structure,protein interaction network,promoter cis-elements. The results showed that there were 14 SKP1-like family members in longan,which were named as DlSKP1-1-DlSKP1-14. The number of amino acids,molecular weight and isoelectric point of proteins encoded by the family genes were 75 to 399 aa,8.29 to 45.34 kD,and 4.44 to 5.76,respectively. They did not contain signal peptides and might be mainly located in chloroplast. There were one pair of tandem repeat genes and four pairs of fragment repeat genes in the DlSKP1 family. The protein interaction prediction results suggested that DlSKP1 family members might interact with multiple proteins(especially F-Box family proteins). The results of the promoter cis-acting elements showed that the promoters of DlSKP1 family members contained more abscisic acid(ABA)and methyl jasmonate(MeJA)response elements. In addition,longan DlSKP1 members had five different expression patterns during early somatic embryogenesis,among which three members(DlSKP1-6,DlSKP1-8,DlSKP1-13)had higher expression levels than other members at each stage. The results of the study indicated that DlSKP1 family members might interact with the F-Box proteins,participate in the regulation of ABA and MeJA,and also play an important role in the morphogenesis of longan somatic embryos.

Key words: Dimocarpus longan, SKP1-like family, geome-wide identification, molecular characteristics, early somatic embryogenesis, expression

CLC Number: